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Methylation of TIMP3 in esophageal squamous cell carcinoma |
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| Authors: | Smith Eric De Young Neville J Tian Zi-Qiang Caruso Maria Ruszkiewicz Andrew R Liu Jun-Feng Jamieson Glyn G Drew Paul A |
| Institution: | 1. Discipline of Surgery,School of Medicine,Faculty of Health Sciences,The University of Adelaide,Royal Adelaide Hospital,South Australia 5005,Australia 2. Department of Thoracic Surgery,Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,Hebei Province,China 3. Division of Tissue Pathology,Institute of Medical and Veterinary Science,Adelaide,South Australia 5000,Australia 4. School of Nursing and Midwifery,Flinders University,Bedford Park,South Australia 5042,Australia |
| Abstract: | AIM: To measure the frequency of DNA methylation of the tissue inhibitor of metalloproteinase 3 (TIMP3)promoter and relate this to any change of gene expression in esophageal squamous cell carcinoma in patients from a region of high incidence in China.METHODS: Cancer cell lines were treated with or without the demethylating reagent 5-aza-2'-deoxycytidine.Methylation of the TIMP3 promoter was assessed in three regions by melt curve analysis and its expression was assessed by real-time RT-PCR. Tumors and proximal resection margins were obtained from 64 patients with esophageal squamous cell carcinoma from a region of high incidence in China. Methylation was assessed by melt curve analysis and expression by immunohistochemistry.RESULTS: Methylation in one of the three promoter regions assessed correlated with gene silencing in esophageal cell lines. A degree of methylation of TIMP3 was found in only four esophageal squamous cell carcinomas, and partial loss of TIMP3 protein expression in just one. |
| Keywords: | Esophageal squamous cell carcinoma Immu-nohistochemistry Methylation TIMP3 |
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