PYK2在海人酸癫痫大鼠海马神经元及小胶质细胞中的表达变化

目的 观察海人酸癫痫大鼠海马内神经元和小胶质细胞中PYK2的表达并探讨其意义。方法 建立海人酸大鼠癫痫模型，大鼠癫痫发作后8、24、72h后处死大鼠，采用免疫组化方法观察大鼠海马内小胶质细胞的活化状况，以及海马神经元和活化后小胶质细胞中PYK2的表达变化。结果 与正常大鼠相比，癫痫大鼠中PYK2的表达在癫痫发作8h后急剧下降，并随时间推移继续下降。癫痫发作24h后，海马中出现大量呈阿米巴形状、强烈表达活化型PYK2的活化型小胶质细胞；癫痫后72h，海马中出现大量有活化型PYK2强表达的“杆”状活化型小胶质细胞。结论 癫痫可致海马神经元及小胶质细胞PYK2表达变化；小胶质细胞中活化型PYK2的表达可能与小胶质细胞活化有关。

Changes of PYK2 expression in hippocampal neurons and microglial cells in Kainate acid-induced status epilepticus of rats

Objective To investigate and evaluate the changes of PYK2 expression in hippocampal neurons and microglial cells in Kainate acid-induced status epilepticus. Methods Kainate acid-induced status epilepticus was established in rats, and by using immunostaining, changes of PYK2 expression in hippocampal neurons and microglial cells was investigated. Results Expression of PYK2 in hippocampal pyramidal neurons was markedly decreased after kainate acid-induced status epilepticus. However, 24h after the epileptic onset, a pronounced up-regulation of PYK2 and phosphor-PYK2 immunoreactivities were evident in amoeboid microglial cells. The upregulation of PYK2 and phosphor-PYK2 was in accordance with the morphological changes of the activated microglial cells. Conclusion PYK2 was activated in microglial cells after seizure. Furthermore, the activation of PYK2 in microglial cells after seizure might be related to the morphological and behavioral changes of microglial cells after activation.