fMRI评价NGF基因治疗对犬脑出血血肿灶周神经功能修复的影响

目的探讨fMRI在评价神经生长因子(NGF)基因治疗犬脑出血疗效中的价值.方法健康家犬24只,随机分为3组:NGF组(n=12),脑出血组(n=8)和对照组(n=4).在脑出血后1天、3天、10天、28天四个时间点进行MR扫描、Purdy评分和透射电镜观察.结果 NGF组1～3天时血肿、水肿体积与脑出血组比较无显著性差异(P>0.05),血肿灶周ADC、rCBF与脑出血组比较有显著性差异(P<0.05).10～28天血肿、水肿体积和血肿灶周ADC、rCBF均显著好于脑出血组(P<0.05),且fMRI动态演变与临床神经功能转归和病理结果相一致.结论 fMRI能客观、有效地定性和定量评价NGF基因治疗脑出血的疗效.

fMRI evaluation of NGF gene therapy on neural functional recovery in the region of perihematoma after intracerebral hemorrhage

Objective To study the applied value of using fMRI to evaluate the therapeutic effect of NGF gene on neural functional recovery in the region of perihematoma after experimental intracerebral hemorrhage (ICH). Methods Twenty-four healthy dogs were divided into three groups randomly: NGF group (n=12), ICH group (n=8) and control group (n=4). The examinations of Purdy score, MR scanning and TEM were carried out at 1, 3, 10, 28 day after ICH. Results Compared with ICH group, the volume of hematoma and edema in NGF group had no significant difference (P>0.05), whereas ADC and rCBF in NGF group had significant difference at 1-3 day (P<0.05). At 10-28 day, the volume of hematoma and edema, ADC and rCBF in NGF group were obviously better than that in ICH group (P<0.05). The dynamic changes of MR were correlated with the changes of clinic neural function and pathological result. Conclusion fMRI was an objective and effective method to evaluate quantitatively and qualitatively the results of gene therapy.