| 胆囊收缩素酶联免疫测定法的应用 |
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| 引用本文： | 陈曼玲,何涛,邱明均.胆囊收缩素酶联免疫测定法的应用[J].四川大学学报(医学版),1999,30(2):7. |
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| 作者姓名： | 陈曼玲 何涛 邱明均 |
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| 作者单位： | 华西医科大学基础医学院,生物化学教研室,成都,610041 |
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| 摘 要： | 对已建立的八肽胆囊收缩素（ＣＣＫ８）酶联免疫吸附试验的应用作初步探索。结果显示：相关肽２Ａｇ、３Ａｇ及ＣＣＫ８之抑制曲线斜率有异，分别为－０．４６、－０．１７、－０．６８；脑组织提取物可被ＣＣＫ８定量抑制；ＣＣＫ８不与其他４种八肽抗血清发生反应，表明该法可用于对ＣＣＫ相关肽进行初步结构分析，亦可用于组织提取物ＣＣＫ成分鉴定及多肽抗血清筛选，因而具有较好的应用前景。
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| 关 键 词： | 胆囊收缩素 酶联免疫吸附试验(ELISA) |
Application of Enzyme-Linkd Immunosorbent Assay for Cholecystokinin |
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| Chen Manling,He Tao,Qiu Mingjun.Application of Enzyme-Linkd Immunosorbent Assay for Cholecystokinin[J].Journal of West China University of Medical Sciences,1999,30(2):7. |
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| Authors: | Chen Manling He Tao Qiu Mingjun |
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| Abstract: | An enzymelinked immunosorbent assay for octapeptide cholecystokinin (CCK) has been developed. Using this method we distinguished two kinds of CCKrelated peptides and is also useful for identified the compounds with CCKlike structure in tissue extracts, as well as different antipeptide antisera. The results showed that this method could be applied to preliminary structure analysis of CCK. |
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| Keywords: | Cholecystokinin enzymelinkedImmunosotrbent assay |
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