| 头体针对局灶性脑缺血大鼠脑组织CREB表达的影响 |
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| 引用本文： | 付雷,易艳东,张唐法,黄浩. 头体针对局灶性脑缺血大鼠脑组织CREB表达的影响[J]. 中国康复, 2011, 26(1): 10-12. DOI: 10.3870/zgkf.2011.01.003 |
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| 作者姓名： | 付雷 易艳东 张唐法 黄浩 |
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| 作者单位： | 武汉中西医结合医院实验中心,武汉,430022 |
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| 基金项目： | 武汉市卫生科研基金项目(No.WX09C01) |
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| 摘 要： | 目的：探讨头体针对局灶性脑缺血大鼠顶叶皮质cAMP反应元件结合蛋白（CREB）和磷酸化CREB（p-CREB）表达的影响。方法：SD雄性大鼠90只,随机分为正常组、模型组及针刺组各30只。模型组及针刺组大鼠采用中脑动脉闭塞（MCAO）法成功制作为局灶性脑缺血模型。针刺组于大鼠清醒后约1 h即平刺百会穴及点刺水沟穴,并连接电刺激;直刺双侧三阴交和丰隆穴,留针30 min,每日1次。治疗15 d后3组均采用神经行为学评分;应用实时定量PCR、Western Blotting和图像分析方法检测大鼠缺血侧顶叶皮质CREB和pCREB的表达。结果：造模后1～2 h及15d时神经症状行为评分,模型组及针刺组均明显高于正常组（P〈0.01）;针刺组在治疗15 d时较治疗前及模型组有明显降低（P〈0.05）,模型组则变化不明显。脑组织CREB的mRNA表达,模型组及针刺组均较正常组明显降低,针刺组明显高于模型组（P〈0.05）。结论：头体针能明显上调局灶性脑缺血大鼠顶叶皮质CREB和pCREB的表达,对缺血神经元有保护作用。
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| 关 键 词： | 头体针 脑缺血 cAMP反应元件结合蛋白 顶叶皮质 |
Effect of Scalp and Body Acupuncture on Expression of CREB and Phosphorylated CREB Protein during Focal Cerebral Ischemia in Rat Parietal Cortex |
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| FU Lei,YI Yan-dong,ZHANG Tang-fa,et al.. Effect of Scalp and Body Acupuncture on Expression of CREB and Phosphorylated CREB Protein during Focal Cerebral Ischemia in Rat Parietal Cortex[J]. Chinese Journal of Rehabilitation, 2011, 26(1): 10-12. DOI: 10.3870/zgkf.2011.01.003 |
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| Authors: | FU Lei YI Yan-dong ZHANG Tang-fa et al. |
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| Affiliation: | FU Lei,YI Yan-dong,ZHANG Tang-fa,et al.Center of Experimental Medicine,Wuhan First Hospital,Wuhan 430022,China |
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| Abstract: | Objective:To explore the effect of scalp and body acupuncture on the expression of cyclic AMP response element binding protein(CREB) and phosphorylated CREB in rat parietal cortex during focal cerebral ischemia.Methods:Ninety male SD rats were randomly divided into control group,model group,and treatment group.Focal cerebral ischemia model was established by occlusion of the middle cerebral artery using the intraluminal suture method.Baihui(GV20) and Shuigou(GV26) were punctured and stimulated by twirling t... |
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| Keywords: | scalp and body acupuncture cerebral ischemia cAMP response element binding protein parietal cortex |
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