| RNA干扰survivin对口腔表皮样癌细胞株 KB生长的抑制作用 |
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| 引用本文： | 李刚,谭晓虹.RNA干扰survivin对口腔表皮样癌细胞株 KB生长的抑制作用[J].肿瘤防治研究,2011,38(3):257-260. |
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| 作者姓名： | 李刚 谭晓虹 |
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| 作者单位： | 1. 梧州市红十字会医院化疗一科,广西梧州,543002
2. 广西医科大学附属肿瘤医院化疗三科 |
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| 基金项目： | 广西壮族自治区科学基金资助项目(0236007) |
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| 摘 要： | 摘要：目的探讨RNA干扰技术沉默survivin基因对口腔表皮样癌细胞株KB增殖和凋亡的影响。方法化学合 成靶向survivin基因的小干扰RNA片段（siRNA），脂质体法转染KB细胞。通过RT-PCR、蛋白印迹法分别检 测survivin mRNA和蛋白表达变化，流式细胞仪检测细胞生长周期和凋亡，MTT法检测转染后KB细胞增殖情 况。结果siRNA可以有效地降低KB细胞survivin mRNA和蛋白的表达；转染后KB细胞阻滞于G2/M期，凋亡增 加，增殖受抑制。结论应用RNA干扰技术沉默survivin 在KB细胞中的表达可以有效抑制该细胞增殖、促进 癌细胞凋亡，将survivin作为口腔癌基因治疗的靶点，通过RNA干扰技术对口腔癌进行基因治疗具有一定 的可行性。
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| 关 键 词： | 关键词：RNA干扰 凋亡抑制因子 口腔癌 凋亡 |
| 收稿时间： | 2010-02-22; |
Inhibitory Effect on Growth of Oral Cancer Cell Lines KB Induced by survivin RNAi |
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| LI Gang,TAN Xiao-Hong.Inhibitory Effect on Growth of Oral Cancer Cell Lines KB Induced by survivin RNAi[J].Cancer Research on Prevention and Treatment,2011,38(3):257-260. |
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| Authors: | LI Gang TAN Xiao-Hong |
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| Institution: | LI Gang1,TAN Xiao-Hong2 1.The First Department of Chemotherapy,Wuzhou Red Cross Hospital,Wuzhou 543002,China,2.The Third Department of Chemotherapy,Tumorical Hospital Affiliated Guangxi Medical University |
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| Abstract: | Objective To observe the inhibitory effect on oral cancer cell lines KB induced by RNA interference(RNAi) of survivin gene.Methods Chemically synthesized small interference RNA(siRNA) targeting survivin was transfected into KB cells by lipofectamine method.The expression of survivin mRNA and protein were detected by RT-PCR and Western blot respectively.The cell cycle and apoptosis were detected by flow cytometry(FCM).The growth of KB cells was detected by MTT.Results The expression of survivin mRNA and prot... |
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| Keywords: | RNA interference survivin Oral cancer cells Apoptosis |
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