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| 四物合剂对马利兰诱导HL-60细胞凋亡的 影响 | |
| 引用本文： | 任亮,张志国,张延新,马菲,杜钢军.四物合剂对马利兰诱导HL-60细胞凋亡的 影响[J].肿瘤防治研究,2011,38(3):261-264. |
| 作者姓名： | 任亮 张志国 张延新 马菲 杜钢军 |
| 作者单位： | 1. 漯河医学高等专科学校药学系,河南漯河,462002 2. 漯河市源汇区妇幼保健院儿科 3. 河南大学药学院 |
| 基金项目： | 漯河医学高等专科学校科研基金资助项目(2009-LMC-S-14); 漯河市科技进步奖励资助项目(2009518) |
| 摘 要： | 目的观察不同剂量四物合剂对马利兰诱导HL-60细胞凋亡作用的影响。方法MTT法检测细胞增殖活性 ，流式细胞术检测细胞凋亡情况，Western blot检测细胞p53/bcl-2表达，ELISA法检测细胞色素C释放， 分光光度法检测Caspase-3、Caspase-8和谷胱甘肽（GSH）。结果四物合剂可激活细胞Caspase-3和 Caspase-8，上调细胞p53/bcl-2表达比率、升高细胞色素C释放、降低GSH（P＜0.05，P＜0.01），且 60mg/L剂量可抑制HL-60细胞生长（P＜0.05）。结论四物合剂可抑制HL-60细胞生长，对马利兰诱导HL-60 细胞凋亡具有增效作用，其增效作用机制可能与激活肿瘤细胞凋亡PKC通路有关。 |
| 关 键 词： | 关键词：四物合剂 马利兰 HL-60细胞 凋亡通路 |
| 收稿时间： | 2010-03-12; |
Effect of Siwu Mixture on Apoptosis Induced by Myleran in HL-60 Leukemic Cells |
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| REN Liang,ZHANG Zhi-guo,ZHANG Yan-xin,MA Fei,DU Gang-jun.Effect of Siwu Mixture on Apoptosis Induced by Myleran in HL-60 Leukemic Cells[J].Cancer Research on Prevention and Treatment,2011,38(3):261-264. | |
| Authors: | REN Liang ZHANG Zhi-guo ZHANG Yan-xin MA Fei DU Gang-jun |
| Institution: | REN Liang1,ZHANG Zhi-guo1,ZHANG Yan-xin1,MA Fei2,DU Gang-jun3 1.Department of Pharmacy,Luohe Medical College,Luohe 462002,China,2.Department of Pediatrics,The Women and Children Health Hospital of Yuanhui District,3.Pharmaceutic College of Henan University |
| Abstract: | Objective To observant the dose effects of Siwu Mixture on the apoptosis in HL-60 leukemic cells by myleran.Methods HL-60 leukemic cells proliferation was tested by MTT assay,cell apoptosis was analyzed by flow cytometry,the p53/bcl-2 expression was assessed by Western blot,cytochrome C release was determined by ELISA,GSH,Caspase-3 and Caspase-8 activation were examined by colorrimetric assay.Results Siwu mixture could increase HL-60 leukemic cell apoptosis by myleran,upregulated ratio of p53/bcl-2 expressi... |
| Keywords: | Siwu mixture Myleran HL-60 Leukemic cells Apoptosis signalway |
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