比较四种冷冻载体在水牛卵母细胞快速玻璃化冷冻保存中的效果

目的探讨不同冷冻载体对水牛卵母细胞快速玻璃化冷冻保存效果的影响。方法以体外成熟培养18 h的水牛卵母细胞为实验材料,分别以0.25 ml冻精塑料细管(A)、自制拣卵针尖端部分制成的极细玻璃微管(D)和两种制作核移植工具针的玻璃细管(B、C)为冷冻载体卵母细胞,解冻后统计成熟率及早期胚胎发育率等冷冻保存效果指标。结果A组卵母细胞冷冻保存效果最差(成熟率:A vs B、C、D为43.9%vs 51.7%、51.9%、55.9%,2~4细胞率:A vs B、C、D为34.1%vs 40.0%、40.7%、41.2%),D组卵母细胞冷冻效果最好,但解冻时卵母细胞的回收率显著低于其他组(D vs A、B、C为69.2%vs 87.0%、94.1%、91.9%)。B、C两组在解冻后的成熟率及早期发育率上显著高于A组,低于D组,但差异不明显,且其解冻后卵母细胞回收率显著高于D组(P<0.05)。结论综合回收率和解冻后早期胚胎发育率,B、C两种玻璃细管是一种水牛卵母细胞快速玻璃化冷冻保存中可行的冷冻载体。

Comparative study of different frozen carriers in fast vitrification preservation of buffalo oocytes

Four different frozen carriers,0.25 ml sperm straw(A),very thin glass capillary tube intercepted from self-made ovum pickup needle(D),and two kinds of thin glass tubes used to make nuclear transplantation needle(B,C),were employed to vitrify in vitro matured buffalo oocytes.The recovery rate,maturation rate and early embryo development of the thawed oocytes were examined.The results showed that,the effect of preservation in A group was the worst(the rate of IVM after thawing: A vs B,C,D was 43.9% vs 51.7%,5...