| 三种不同严重程度大鼠重症胰腺炎模型比较研究 |
| |
| 引用本文： | 袁琳,周秉舵,王晓素.三种不同严重程度大鼠重症胰腺炎模型比较研究[J].中国比较医学杂志,2015,25(5):20-23. |
| |
| 作者姓名： | 袁琳 周秉舵 王晓素 |
| |
| 作者单位： | 上海中医药大学科技实验中心, 上海 201203;上海中医药大学附属岳阳中西医结合医院消化科, 上海 200437;上海中医药大学附属岳阳中西医结合医院消化科, 上海 200437;上海中医药大学科技实验中心, 上海 201203;上海中医药大学附属岳阳中西医结合医院消化科, 上海 200437;上海中医药大学附属岳阳中西医结合医院消化科, 上海 200437;上海中医药大学附属岳阳中西医结合医院消化科, 上海 200437;上海中医药大学附属岳阳中西医结合医院消化科, 上海 200437;上海中医药大学附属岳阳中西医结合医院消化科, 上海 200437;上海中医药大学附属岳阳中西医结合医院消化科, 上海 200437;上海中医药大学附属岳阳中西医结合医院消化科, 上海 200437;上海中医药大学附属岳阳中西医结合医院消化科, 上海 200437;上海中医药大学附属岳阳中西医结合医院消化科, 上海 200437 |
| |
| 基金项目： | 国家自然科学基金青年基金(81302929);上海市教委科研创新项目(14YZ065);上海市杏林新星计划(ZYSNXD011-RC-XLXX-20130019)。 |
| |
| 摘 要： | 目的 为研究者选择合适的牛磺胆酸钠浓度来制备大鼠重症胰腺炎(severe acute pancreatitis,SAP)SAP模型提供依据。方法 将60只SD大鼠随机分为假手术组、1.5%浓度组、3.5%浓度组和5%浓度组, 造模各组分别用1.5%、3.5%和5%牛磺胆酸钠按逆行胆胰管注射法制备SAP模型。术后统计各组大鼠的死亡率;检测血清淀粉酶、肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)水平;观测各组大鼠胰腺组织HE染色病理评分。结果 5%浓度组死亡率较1.5%浓度组显著升高, 血淀粉酶、肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)水平、出血和腺泡组织坏死的病理评分较1.5%浓度组和3.5%浓度组均有显著升高。结论 5%的牛磺胆酸钠逆行胆胰管注射法能更好的制备SAP模型, 且更符合SAP的生理、病理表现。
|
| 关 键 词： | 重症胰腺炎 逆行胆胰管注射法 牛磺胆酸钠 模型 动物 |
| 收稿时间： | 2014/11/14 0:00:00 |
| 修稿时间： | 2015/4/21 0:00:00 |
Comparative study of SAP model in rats with three different severity |
| |
| YUAN LIN,ZHOU BING DUO and WANG XIAO SU.Comparative study of SAP model in rats with three different severity[J].Chinese Journal of Comparative Medicine,2015,25(5):20-23. |
| |
| Authors: | YUAN LIN ZHOU BING DUO and WANG XIAO SU |
| |
| Institution: | Shanghai University of T.C.M Experiment Center for Science and Technology, Shanghai 201203, China;Department of Gastroenterology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China;Department of Gastroenterology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China;Shanghai University of T.C.M Experiment Center for Science and Technology, Shanghai 201203, China;Department of Gastroenterology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China;Department of Gastroenterology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China;Department of Gastroenterology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China;Department of Gastroenterology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China;Department of Gastroenterology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China;Department of Gastroenterology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China;Department of Gastroenterology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China;Department of Gastroenterology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China;Department of Gastroenterology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China |
| |
| Abstract: | |
| |
| Keywords: | Severe acute pancreatitis The method of retrograde injection into the biliopancreatic duct Sodium taurocholate Model animal |
|
| 点击此处可从《中国比较医学杂志》浏览原始摘要信息 |
| 点击此处可从《中国比较医学杂志》下载免费的PDF全文 |
|
