| 差异表达新基因BQ135232 ORF的分子克隆与真核表达 |
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| 引用本文： | 马富玲,王海涛,李振莉,刘淑芬,张淑敏,畅继武.差异表达新基因BQ135232 ORF的分子克隆与真核表达[J].肿瘤研究与临床,2007,19(4):221-224. |
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| 作者姓名： | 马富玲 王海涛 李振莉 刘淑芬 张淑敏 畅继武 |
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| 作者单位： | 1. 300211,天津市泌尿外科研究所肿瘤免疫研究室
2. 天津大学药学研究院联合实验室 |
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| 摘 要： | 目的 研究差异表达新基因BQ135232的结构功能以及真核表达。方法 应用生物信息学方法分析BQ135232 mRNA基因序列，初步了解其基本性状，构建表达载体，转染真核细胞（CHO/dhfr-）。结果 BQ135232具备作为免疫原的基本条件，构建表达载体pcDNA3-BQ-ORF-GPI，成功转染CHO/dhfr-。结论 完成差异表达新基因BQ135232的真核表达，为后续的蛋白功能研究奠定基础。
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| 关 键 词： | 计算生物学 新基因 克隆 分子 |
| 收稿时间： | 2006-9-1 |
The cloning and expressing of novel differential expression gene BQ135232 ORF |
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| MA Fu-ling,WANG Hai-tao,LI Zhen-li,LIU Shu-fen,ZHANG Shu-min,CHANG Ji-wu.The cloning and expressing of novel differential expression gene BQ135232 ORF[J].Cancer Research and Clinic,2007,19(4):221-224. |
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| Authors: | MA Fu-ling WANG Hai-tao LI Zhen-li LIU Shu-fen ZHANG Shu-min CHANG Ji-wu |
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| Institution: | Oncological Immune Research Laboratory, Institute of Urological Surgery, Tianjin City, Tianjin |
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| Abstract: | Objective To study the architecture, function, and expression of differential expression novel gene BQ135232 obtained by SSH. Methods By using bioinformatic tools, we primarily understood the basic characters of the novel gene BQ135232, then constructed expressing vector, and transfected in eucaryot－ic cell. Results The bioinformatic analysis indicated that BQ135232 possessed the possibility to be an im－munogen, subsequently we constructed the expressing vector pcDNA3-BQ-ORF-GPI, transfected CHO/dhfr- successfully, and verified by RT-PCR. Conclusion Accomplished the expression of novel gene BQ135232 ORF in eucaryotic cell, laid the groundwork for future protein function research. |
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| Keywords: | Novel gene Cloning molecular |
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