Evaluation of a commercially available serologic assay for antibodies against tuberculosis-associated glycolipid antigen. |
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Authors: | Yoshitsugu Iinuma Kazuyoshi Senda Shunji Takakura Satoshi Ichiyama Masao Tano Tomoji Abe Tomoko Yamamoto Kazumitsu Nakashima Hisashi Baba Yoshinori Hasegawa Kaoru Shimokata |
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Affiliation: | Department of Clinical Laboratory, Nagoya University Hospital, Nagoya-city, Japan. yiinuma@med.nagoya-u.ac.jp |
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Abstract: | A commercially available enzyme immunoassay developed to detect antibodies to a tuberculosis-associated glycolipid antigen was evaluated for serologic diagnosis of tuberculosis. This was a multicenter study comparing the assay with other methods in 78 patients with active pulmonary tuberculosis and in 54 controls with non-tuberculous lung diseases. Sensitivities were highest for sputum culture (91.0%), followed by immunoassay (79.5%), nucleic acid amplification (77.3%), and finally acid-fast staining of sputum smear (60.3%). Immunoassay and amplification, both rapid methods, had similarly high sensitivity in smear-positive subjects (89.4 and 88.9%, respectively); in smear-negative subjects these two techniques showed low sensitivity (64.5 and 60.0%, respectively). Concordance between the two methods was relatively low (72.0%). With regard to specificity, seven out of ten patients with old tuberculosis had positive result by immunoassay (30% specificity). In the control group, 10 out of 54 patients had positive immunoassay result (72.2% specificity), with notably limited specificity in the elderly. The tuberculous glycolipid assay is a rapid method sufficiently sensitive for detection of tuberculosis infection, even in smear-negative patients. |
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