辐射对脑损害的晚期效应及中药的保护作用

目的　了解小鼠脑组织放射后的晚期损害 (智力、生存率、生存质量 )及寻找保护药物。方法　将 6 0只BALB/C小鼠分为对照和不同处理共 5组 ,各组全脑单次照射 2 2Gy ,观测各组小鼠体重及生存情况 ,小鼠学习和记忆功能的改变 ,组织病理学改变 ,比较组间差别。结果　各用药组小鼠生存率无统计学差异 ;丹参组能明显改善小鼠的学习和记忆功能 ,组间有统计学差异 ;丹参组对放射诱导的小鼠脑组织老年斑和神经原纤维缠结有明显的抑制作用。结论　所选药物复方丹参明显改善小鼠学习和记忆功能 ,对放射诱导脑组织损害的保护有病理学上的证据 ,但未能提高小鼠生存率。

Radiation-induced late brain injury and the protective effect of traditional Chinese medicine

Objective To investigate whether radiation-induced late injury of the brain can be ameliorated by traditional Chinese Medicine through blocking the primary events. Methods This trial included five animal groups: sham irradiation, irradiation only, and three treatment groups. The whole brain of BALB/C mouse was irradiated with 22?Gy by using a 6?MV linear accelerator. Step down method was used to evaluate the study and memory abilities. Mouse weight was also recorded every week before and after irradiation. On D90, all mice alive were euthanized and Glee's silver dye method and Bielschousky silver dye method were used to detect the senile plaque and the neurofibrillary tangle. One-Way ANOVA was used to evaluate the differences among the groups in the various aspects of study and memory abilities as well as quality of life. Kaplan-Meier was used to evaluate the survival. Log-rank was used to detect the differences among the survival groups. Results 1. There was no significant difference in survival among the treatment groups, even though Salvia Miltiorrhiza (SM) was able to improve the quality of life. As to the cognition function, it was shown that whole brain radiation would make a severe cognition damage with the learning and memorizing ability of the irradiated mice being worse than those of the sham irradiation group. The Traditional Chinese Medicine Salvia Miltiorrhiza possesses the role of a protective agent against cognition function damage induced by irradiation. 2. Glee's silver dye and Bielschousky silver dye show much more senile plaque and the neurofibrillary tangle in brain tissue of R group and R 654-2 group than those in the R SM group. Conclusions Salvia Miltiorrhiza is able to protect the mouse from cognition function damage induced by irradiation and improve the quality of life by ameliorating the primary events, though it does not improve the survival.