Enzyme-like immunosorbent assay (ELISA) for immunoglobulin G antibodies against insect venoms |
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| Authors: | J A Grant R M Goldblum R Rahr D O Thueson J Farnam J Gillaspy |
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| Affiliation: | 1. Departments of Internal Medicine, Pediatrics, and Preventive Medicine and Community Health, University of Texas Medical Branch at Galveston, Galveston, Texas, USA;2. Department of Biology, Texas A & I University at Kingsville, Kingsville, Texas, USA |
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| Abstract: | IgG "blocking" antibodies were measured in patients receiving insect venom immunotherapy. The enzyme-linked immunosorbent assay (ELISA) described herein was found to be sensitive and reproducible. Results with ELISA correlated well with values obtained with a radioimmunoassay and with inhibition of the release of histamine from sensitive basophils. Also, specific antibody titers against phospholipase A and whole bee venom were correlated. Serial determinations of venom-specific IgG antibodies were made in 17 patients receiving Polistes wasp or bee venom immunotherapy. The majority of patients showed a rise in IgG antibodies, which peaked after administration of approximately 500 micrograms of venom. Only one out of 13 of these venom-treated patients had allergic symptoms after an insect sting while on maintenance therapy. |
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| Keywords: | Reprint requests to: J. Andrew Grant M.D. University of Texas Medical Branch Clinical Sciences Building Room 405 Galveston TX 77550. |
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