| 多孔载因子CPC促进骨缺损愈合的实验研究 |
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| 引用本文： | 余雷,刘兴炎,厉孟,葛宝丰,梁建平. 多孔载因子CPC促进骨缺损愈合的实验研究[J]. 中国矫形外科杂志, 2010, 18(8): 673-677 |
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| 作者姓名： | 余雷 刘兴炎 厉孟 葛宝丰 梁建平 |
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| 作者单位： | 兰州军区总医院,兰州,730050 |
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| 基金项目： | 全军“十一五”医药卫生科研基金资助项目(编号:06MA090); 甘肃省自然科学基金资助项目(编号:0710RJZA068) |
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| 摘 要： | [目的]探讨磷酸钙骨水泥复合rhBMP-2/明胶微球复合材料在治疗骨缺损时的降解、成骨性能。[方法]制备携载rhBMP-2的明胶微球(GMs),与磷酸钙骨水泥(CPC)复合,制备出rhBMP-2/GMs/CPC复合人工骨。取30只新西兰大白兔,在前臂桡骨中段制造人工骨缺损,随机分成3组,分别植入rhBMP-2/GMs/CPC/复合物(A组)、GMs/CPC(B组)、rhBMP-2/CPC(C组),术后6、12周分别进行X线检测、骨密度测定,术后12周处死动物,分别行生物力学测定,脱钙切片、HE染色,不脱钙切片进行荧光显微镜下观察双标间距,计算平均矿化率。[结果]与GMs/CPC、rhBMP-2/CPC组比较,复合材料植入后不同时间点的材料降解及成骨均高于对照组。12周A组标本生物力学实验测定结果表明指标接近正常,与B、C组比较有统计学差异。骨密度12周、新骨矿化率提示有统计学差异。[结论]rhBMP-2/GMs/CPC微球系统复合材料在体内易降解,具有良好成骨活性,是良好的骨修复材料。
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| 关 键 词： | 磷酸钙骨水泥 明胶微球 重组人骨形成蛋白-2 降解 骨替代 骨缺损 |
Experimental investigations of macroporous calcium phosphate cements/bone morphogenic protein composite in repairing bone defects |
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| YU Lei,LIU Xing-yan,LI Meng,et al.. Experimental investigations of macroporous calcium phosphate cements/bone morphogenic protein composite in repairing bone defects[J]. Orthopedic Journal of China, 2010, 18(8): 673-677 |
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| Authors: | YU Lei LIU Xing-yan LI Meng et al. |
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| Affiliation: | YU Lei,LIU Xing-yan,LI Meng,et al.Orthopedic Department,Xijing Hospital,The Fourth Military Medical University,Xi'an 710032,China |
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| Abstract: | [Objective]To analyze the biodegradation properties and active osteogenesis features of implanted composites of gelatin microspheres(GMs) impregnated with calcium phosphate cement(CPC) and recombinant human bone morphogenetic protein-2(rhBMP-2) in bone defect.[Method]rhBMP-2 loaded gelatin microspheres was incorporated in rhBMP-2/CPC during setting.Thirty rabbits were divided into groups A,B and C(10 each).The rhBMP-2/GMs /CPC,GMs/cpc and rhBMP-2/cpc was implanted into the rabbits of group A,B and C,respect... |
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| Keywords: | calcium phosphate cement gelatin microspheres bone morphogenetic protein biodegradation bone substitute bone defect |
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