Decreased serum DNase1-activity in patients with autoimmune liver diseases |
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| Authors: | Nikolaos K Gatselis Aigli G Vakrakou Kalliopi Zachou Theodoros Androutsakos Kalliopi Azariadis Gregorios Hatzis |
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| Institution: | 1. Department of Medicine and Research Laboratory of Internal Medicine, School of Medicine, University of Thessaly, Larissa, Greece,;2. Department of Pathophysiology, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece,;3. Department of Molecular Medicine, Hellenic Pasteur Institute, Athens, Greece, and;4. Department of Pathophysiology, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece, |
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| Abstract: | Deoxyribonuclease1 (DNase1) is involved in chromatin degradation of apoptotic cells. Its deficiency results in accumulation of self-DNA, which in turn may induce inflammation and autoimmunity. We assessed for the first time serum DNase1-activity in a large consecutive cohort of treatment-naïve patients with autoimmune liver diseases (ALD). DNase1-activity was determined by single radial enzyme-diffusion (SRED) at diagnosis of 224 patients with autoimmune hepatitis (AIH), 249 with primary biliary cirrhosis (PBC) and 36 with primary sclerosing cholangitis (PSC). Sera from 146 patients with chronic hepatitis B or C, 140 with nonalcoholic fatty liver disease/nonalcoholic steatohepatitis (NAFLD/NASH) and 114 healthy individuals served as disease and healthy controls. Available serum samples during remission from 50 AIH and 39 PBC patients were also investigated by paired analyzes. DNase1-activity was significantly lower in AIH, PBC and PSC compared to viral hepatitis (p?p?p?=?0.03), NAFLD/NASH (p?p?p?p?1400?mg/dl; p?p?p?p?=?0.008). In PSC, DNase1-activity was inversely associated with alkaline phosphatase (ALP) (p?p?p?
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| Keywords: | Liver autoimmunity deoxyribonuclease1 apoptosis autoimmune hepatitis primary biliary cirrhosis primary sclerosing cholangitis |
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