喉咽鳞癌患者外周血T淋巴细胞亚群和NK细胞活性分析

背景与目的:T淋巴细胞亚群及NK细胞是主要的细胞免疫形式,通过对两者的研究可以了解细胞免疫在肿瘤发生、发展的作用。喉咽鳞癌患者可能存在一定程度的细胞免疫缺陷,本研究是通过检测外周血T淋巴细胞亚群及NK细胞活性,了解喉咽鳞癌患者的细胞免疫功能。方法:用流式细胞仪对78例喉咽鳞癌患者的外周血进行T淋巴细胞亚群及NK细胞活性的检测,并取20例非肿瘤患者的外周血作为对照组。结果:喉咽鳞癌患者组较非肿瘤患者组CD4淋巴亚群、CD4/CD8比值下降,CD8淋巴亚群升高;NK细胞活性下降。T3～4组较T1～2组、N 组较N0组都有CD4淋巴亚群、CD4/CD8比值下降;NK细胞活性下降。中低分化组CD4淋巴亚群、CD4/CD8比值下降(均P<0.05)。结论:喉咽鳞癌患者CD4T淋巴细胞亚群及NK细胞活性受到抑制,细胞免疫功能降低;检查外周血T淋巴细胞亚群及NK细胞活性有助于喉咽鳞癌患者的细胞免疫功能监测。

Analysis of serum T-lymphocyte subsets and NK cell activity in patients with hypopharyngeal squamous cell carcinoma

BACKGROUND & OBJECTIVE: T-lymphocyte subsets and NK cell are the major forms of cellular immunity. Study of these 2 types of cells may lead to the better understanding of the function of cellular immunity in the onset and development of carcinoma. To a certain degree, there may be cellular immunodeficiency existing in patients with hypopharyngeal squamous cell carcinoma. This study was to investigate the cellular immunity function in these patients. METHODS: T-lymphocyte subsets and NK activity were determined by flow cytometry in 78 patients with hypopharyngeal squamous cell carcinoma. Blood samples of 20 non-tumor patients were used as control. RESULTS: The levels of CD4 lymphocyte subsets, CD4/CD8 ratio, and NK activity were lower in carcinoma group than in control group, but CD8 lymphocyte level was higher in carcinoma group. The levels of CD4 lymphocyte subsets, CD4/CD8 ratio, and NK activity were lower in T3-4 group than in T1-2 group, and lower in N+ group than in N0 group. The levels of CD4 lymphocyte subsets and CD4/CD8 ratio were decreased in the carcinoma with moderate or low differentiation (P<0.05). CONCLUSIONS: T-lymphocyte subsets and NK activity are inhibited, and the cellular immunology is suppressed in the patients with hypopharyngeal squamous cell carcinoma. Analyzing T-lymphocyte subsets and NK activity would be helpful to evaluate the cellular immunologic condition of these patients.