| 聚合酶链反应和杂交试验检测上海地区庚型肝炎病毒感染的初步报告 |
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| 引用本文： | 金根娣,陆志檬,张东华,邱纪慧,张欣欣,陆林,陈竺.聚合酶链反应和杂交试验检测上海地区庚型肝炎病毒感染的初步报告[J].中华传染病杂志,1997(3). |
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| 作者姓名： | 金根娣 陆志檬 张东华 邱纪慧 张欣欣 陆林 陈竺 |
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| 作者单位： | 上海第二医科大学附属瑞金医院，上海市血液学研究所 |
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| 摘 要： | 为探讨上海地区庚型肝炎病毒感染的现状，采用逆转录套式聚合酶链反应（ＲＴＮｅｓｔｅｄＰＣＲ）检测庚型肝炎病毒（ＨＧＶ／ＧＢＶＣ）核酸（ＨＧＶＲＮＡ）。结果在各类患者和助血员中ＨＧＶＲＮＡ的检出率分别是：血液透析和肾移植为１５７％（８／５１）、丙型肝炎为３３％（１／３０）、乙型肝炎为０（０／１９）、散发性非Ａ～Ｅ型肝炎为０（０／２８）、义务助血员为７５％（５／６７）。提示ＨＧＶ感染多为无症状或亚临床型，常与ＨＣＶ重叠感染，并与输血密切相关。作者肯定了上海地区存在庚型肝炎，指出筛选助血员对控制输血后庚型肝炎至关重要。
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| 关 键 词： | 肝炎病毒.庚型 聚合酶链反应 核酸杂交 |
Initial report on the detection of hepatitis G virus infection in Shanghai using RT-Nested-PCR and hybridization |
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| Jin Gendi,Lu Zhimeng,Zhang Donghua,et al..Initial report on the detection of hepatitis G virus infection in Shanghai using RT-Nested-PCR and hybridization[J].Chinese Journal of Infectious Diseases,1997(3). |
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| Authors: | Jin Gendi Lu Zhimeng Zhang Donghua |
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| Institution: | Jin Gendi,Lu Zhimeng,Zhang Donghua,et al. Clinical Virology Research Unit,Ruijin Hospital,Shanghai Second Medical Univensity,200025 |
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| Abstract: | In order to study hepatitis G virus (HGV) infection in Shanghai,Nested RT-PCR has been established to detect RNA of HGV/GBV-C according to literature.Results showed the positive detection rate was 15.7%(8/51),3.3%(1/30),0(0/19) and 0(0/28) in patients with hemodialysis or kidney transplation,hepatitis C,hepatitis B and hepatitis Non A-E respectively as well as 7.5%(5/67)of blood donors were found positive for HGV/GBV-C RNA..The clinical manifestation of HGV infection was generally mild or subclinical.HGV infection had a close correlation with blood transfusion and may be coinfected with HCV.There was a high infection rate among blood donors.The existence of HGV infection in Shanghai could be affirmed and it is suggested that screening blood donors is of prime importance for the prevention of post-transfusion bepatitis G. |
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| Keywords: | Hepatitis G virus Polymerase Chain Reaction Nucleic acid hybridization |
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