荧光原位杂交检测慢性粒细胞白血病的染色体畸变

目的 检测费城染色体（Ｐｈｉｌａｄｅｌｐｈｉａ ｃｈｒｏｍｏｓｏｍｅ，Ｐｈ染色体）阴性，变异型Ｐｈ染色体及伴有其它染色体异常的慢性粒细胞白血病（ｃｈｒｏｎｉｃ ｍｙｅｌｏｉｄ ｌｅｕｋｅｍｉａ，ＣＭＬ）的ｂｃｒ／ａｂｌ融合基因。方法 双色荧光原位杂交技术，检测伴有８种不同的骨髓细胞染色体畸变ＣＭＬ患者的ｂｃｌ／ａｂｌ融合基因。结果 ３例变异型Ｐｈ和７例有标准型Ｐｈ的ＣＭＬ患者均为ｂｃｒ／ａｂｌ融合

A moleucular cytogenetic study on chromosome anomalies of chronic myeloid leukemia

Objective To study the bcr/abl fusion gene in chronic myeloid leukemia (CML) cases with Philadelphia chromosome(Ph)negative,and variant Ph translocations and other chromosome anomalies.Methods The status of bcr/abl fusion gene in 8 kinds of chromosome anomalies was tested with dual color fluorescence in situ hybridization(D FISH) technique. Results The positive hybridization was demons trated in 3 cases of variant Ph CML and other 7 cases of standard Ph, respectively. In 2 cases of Ph negative CML, one case that had karyotype such as 46,XY,t(9;18) showed positive hybridization, the other case with 46,XY, inv(2) showed negative result. Conclusion The result demonstrates that D FISH technique is reliable for detecting bcr/abl fusion gene. The existence of negative bcr/abl fusion gene CML implies that some other initial events inducing CML might have occurred in such cases.