浸润性乳腺癌中BCSG1的表达及临床意义

目的探讨乳腺癌特异基因1（BCSG1）在乳腺癌组织中的表达及其临床意义。方法分别采用荧光实时定量PCR（FQ-PCR）和免疫组织化学方法检测51例乳腺癌组织和35例癌旁组织中BCSG1的表达。结果FQ—PCR检测乳腺癌组织中BCSG1 mRNA的表达水平为1．072±0．178，相应癌旁组织中其表达量为0．324±0．135；免疫组化检测乳腺癌组织中BCSG1蛋白阳性率为74．5％（38／51），相应癌旁组织巾为14．3％（5／35）；癌组织中BCSG1 mRNA和蛋白的表达均显著高于癌旁组织（P〈0．05）。BCSG1 mRNA和蛋白的表达与淋巴结转移及Nottingham预后指数等参数差异有统计学意义（P〈0．05），但与乳腺癌的病理类型、雌激素受体（ER）、孕激素受体（PR）及C—erbB-2的阳性表达等参数差异无统计学意义（P〉0．05）。结论BCSG1在乳腺癌组织中高表达，与乳腺癌的发展及临床预后密切相关，BCSG1很可能存乳腺癌的发病机制中起重要作用。

Expression and clinical pathological significance of BCSG1in invasive breast cancer

Objective To investigate the expression of breast cancer specific gene 1 （ BCSG1 ） in breast eaneer tissues and its clinical significance. Methods The expression of BCSG1 in 51 cases of breast cancer tissues and 35 cases of adjacent tissues was determined by fluorescence quantitative polymerase chain reaction （FQ-PCR） and immunohistochemistry. Results FQ-PCR showed the expression level of BCSG1 mRNA in breast cancer and adjacent tissues was 1. 072 ± 0. 178 and 0. 324 ± 0. 135 respectively. Immunohistochemistry showed the expression rate of BCSG1 protein in breast cancer and adjacent tissues was 74. 5% （38/51） and 14.3% （5/35）. The expression of BCSG1 mRNA and protein was dramatically increased in breast cancer tissues compared with that in normal background breast tissues （P 〈 0. 05 ）. BCSG1 mRNA and protein expression was significantly different in patients with lymph node metastasis and higher Nottingham prognostic index （P 〈 0.05 ）, and there was no significant difference with the pathological type of breast cancer, or status of ER, PR, and c-erbB2 （P 〉0. 05）. Conclusions BCSG1 is highly expressed in breast cancer tissues, which is closely related to the development and clinical prognosis of breast cancer. BCSG1 is very likely to play an important role in the pathogenesis of breast cancer.