| 天然冰片对小鼠脑内氨基酸类神经递质含量的影响 |
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| 引用本文： | 李伟荣,陈瑞玉,黄天来,许庆文,宓穗卿,王宁生.天然冰片对小鼠脑内氨基酸类神经递质含量的影响[J].中药新药与临床药理,2011,22(2). |
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| 作者姓名： | 李伟荣 陈瑞玉 黄天来 许庆文 宓穗卿 王宁生 |
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| 作者单位： | 广州中医药大学临床药理研究所,广州,510405 |
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| 基金项目： | 国家自然科学基金(30701100); 广州中医药大学师资队伍建设科研项目(A1033007) |
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| 摘 要： | 目的研究小鼠口服天然冰片后脑内冰片含量的变化以及对氨基酸类神经递质含量的影响。方法小鼠灌胃天然冰片,于给药前及给药后0.083,0.167,0.25,0.333,0.5,0.75,1,1.5,2,2.5,3,4 h分别取脑,采用GC-MS法测定冰片含量,HPLC-FLD法测定氨基酸类神经递质含量。结果小鼠口服天然冰片1.2 g/kg后5 min开始出现兴奋症状,持续10~15 min转为抑制状态。天然冰片给药后5 min即可在脑内测得,60 min达到峰值,消除半衰期1.66 h,AUC为326.7μg.h.g-1。脑内天门冬氨酸从给药后0.083 h至1 h含量显著增加,谷氨酸在给药后0.333 h含量显著增加,在给药后1.5 h至4 h含量显著降低,γ-氨基丁酸从给药后0.167 h至4 h含量均显著增加,甘氨酸含量无明显变化。结论天然冰片对中枢神经系统具有兴奋/抑制的双向作用,其作用机制与其对中枢兴奋/抑制性氨基酸类神经递质释放的调控作用有关。
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| 关 键 词： | 天然冰片 天门冬氨酸 谷氨酸 甘氨酸 γ-氨基丁酸 |
Effect of Natural Borneol on Cerebral Amino Acid Neurotransmitters Contents in Mice |
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| LI Weirong,CHEN Ruiyu,HUANG Tianlai,XU Qingwen,MI Suiqing,WANG Ningsheng.Effect of Natural Borneol on Cerebral Amino Acid Neurotransmitters Contents in Mice[J].Traditional Chinese Drug Research & Clinical Pharmacology,2011,22(2). |
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| Authors: | LI Weirong CHEN Ruiyu HUANG Tianlai XU Qingwen MI Suiqing WANG Ningsheng |
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| Institution: | LI Weirong,CHEN Ruiyu,HUANG Tianlai,XU Qingwen,MI Suiqing,WANG Ningsheng(Institute of Clinical Pharmacology,Guangzhou University of Chinese Medicine,Guangzhou 510405,China) |
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| Abstract: | Objective To study the changes of cerebral borneol content and amino acid neurotransmitters contents in mice after oral administration of natural borneol.Methods Mice were administered natural borneol by intragastric gavage.Before administration and 0.083,0.167,0.25,0.333,0.5,0.75,1,1.5,2,2.5,3 and 4 h after administration,mice brain were sampled to determine borneol content by GC-MS and amino acid neurotransmitters contents by HPLC-FLD.Results Excitation symptoms appeared 5 min after oral administration of... |
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