| 五味子多糖对环磷酰胺诱导的小鼠胸腺细胞凋亡的保护作用 |
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| 引用本文： | 许珂玉,柳春.五味子多糖对环磷酰胺诱导的小鼠胸腺细胞凋亡的保护作用[J].中药新药与临床药理,2011,22(2). |
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| 作者姓名： | 许珂玉 柳春 |
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| 作者单位： | 辽宁医学院,长春,110032 |
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| 基金项目： | 辽宁省教育厅基金资助项目(2009A469) |
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| 摘 要： | 目的探讨五味子多糖对环磷酰胺(Cyclophosphamide,CY)诱导小鼠胸腺细胞凋亡的保护作用及相关机制。方法小鼠腹腔注射CY,建立胸腺细胞凋亡模型,施以不同浓度五味子多糖灌胃,利用流式细胞仪、末端转移酶介导的缺口末端标记法(TUNEL染色)及Bcl-2、Bax免疫组化分别观察不同剂量的五味子多糖对CY诱导的胸腺细胞凋亡的影响及机制。结果流式细胞仪和TUNEL染色显示CY组与对照组相比的细胞凋亡率明显增高,五味子多糖治疗组与CY组比较凋亡率明显降低(P<0.01)。CY组bcl-2基因表达明显下降,bax基因表达明显增加,Bcl-2/Bax比值下降。五味子多糖组与之相反,二者比值升高(P<0.01)。结论五味子多糖能够抑制CY诱导的胸腺细胞凋亡,降低胸腺细胞的损伤,其抑制作用可能通过促进Bcl-2表达、抑制Bax蛋白表达实现的。
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| 关 键 词： | 胸腺细胞 细胞凋亡 五味子多糖 环磷酰胺 小鼠 |
Protective Effect of Polysaccharide from Fructus Schisandrae on Cyclophosphamide-induced Thymocyte Apoptosis in Mice |
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| XU Keyu,LIU Chun.Protective Effect of Polysaccharide from Fructus Schisandrae on Cyclophosphamide-induced Thymocyte Apoptosis in Mice[J].Traditional Chinese Drug Research & Clinical Pharmacology,2011,22(2). |
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| Authors: | XU Keyu LIU Chun |
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| Institution: | XU Keyu,LIU Chun(Liaoning Medical College,Changchun 110032,China) |
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| Abstract: | Objective To investigate the protective effect of polysaccharide from Fructus Schisandrae on thymocyte apoptosis induced by cyclophosphamide(CY) in mice and to explore its possible mechanism.Methods Thymocyte apoptosis model was established in mice by intraperitoneal injection of CY.After treatment with different dosages of polysaccharide from Fructus Schisandrae,thymocyte apoptosis was detected by flow cytometry,TUNEL staining,and immunocytochemistry.Results CY-induced group had a higher apoptotic rate tha... |
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| Keywords: | Thymocytes Apoptosis Polysaccharide from Fructus Schisandrae Cyclophosphamide Mice |
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