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| 同一色谱条件下测定黑龙江产山里红叶三种黄酮成分的实验研究 | |
| 引用本文： | 于晓瑾,张妍,刘莉,于翔龙.同一色谱条件下测定黑龙江产山里红叶三种黄酮成分的实验研究[J].中医药信息,2011,28(2):95-97. |
| 作者姓名： | 于晓瑾 张妍 刘莉 于翔龙 |
| 作者单位： | 1. 黑龙江中医药大学,黑龙江哈尔滨150040;哈尔滨工业大学,黑龙江哈尔滨150001 2. 黑龙江省中医研究院,黑龙江哈尔滨,150036 3. 兰格药业,黑龙江哈尔滨,150036 |
| 基金项目： | 黑龙江省科技攻关项目(GB01C107-01); 黑龙江中医药大学校基金(200515) |
| 摘 要： | 目的：建立山里红叶提取物中牡荆素鼠李糖苷、金丝桃苷、芦丁含量测定的方法。方法：采用高效液相色谱法，用Hypersil ODS2 C18色谱柱（5μm，5mm×200mm），以甲醇-0．1％磷酸溶液（40：60）为流动相，流速1．0mL·min^-1，检测波长359nm，柱温35℃。结果：该法线性关系良好，平均加样回收率为牡荆素鼠李糖苷101．2％（RSD=2．3％），金丝桃苷96．9％（RSD=1．8％），芦丁98．9％（RSD=2．6％）（n=6）。结论：该法操作简单，分离效果好，结果准确，专属性强，可作为有效的含量测定方法。 |
| 关 键 词： | 山里红叶 高效液相色谱 牡荆素鼠李糖苷 金丝桃苷 芦丁 |
The Experimental Research on Three Flavone Constituents of Crataegus Pinnatifida Leaves from Heilongjiang Province under the Same Chromatography Condition |
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| YU Xiao-jin,ZHANG Yan,LIU Li,YU Xiang-long.The Experimental Research on Three Flavone Constituents of Crataegus Pinnatifida Leaves from Heilongjiang Province under the Same Chromatography Condition[J].Information on Traditional Chinese Medicine,2011,28(2):95-97. | |
| Authors: | YU Xiao-jin ZHANG Yan LIU Li YU Xiang-long |
| Institution: | YU Xiao-jin1,2,ZHANG Yan3,LIU Li4,YU Xiang-long3 (1.Heilong jiang University of Chinese Medicine,Harbin 150040,China,2.Harbin Institute of Technology,Harbin 150001,3.Heilongjiang Institute of Traditivnal Chinese Medicine,Harbin 150036,4.Harbin Lange Pharmaceutical Limited Company,China) |
| Abstract: | Objective ： To establish the determination method of the vitexin, hyperin and rutin in the leaf of Crataegus pinnatifida Bge. Var. major N. E. Br. extracting solution. Method： The analysis was performed on a Hypersil ODS2-C18 column（5 μm,5mm × 200mm） with methanol - 0. 1% phosphoric acid（40： 60） as mobile phase at a flow rate of 1.0mL·min^-1, and at a column temperature of 35℃. The detection wavelength was 360nm. Results：The linearity of this method was good. The average recovery of added samples was vitexin 101.2% with RSD 2.3% ,hyperin 96. 9% with RSD 1.8% and rutin 98.9% with RSD 2.6% （n =6） . Conclusion ： The method is convenient with a good separating degree for the determination of flavonoids and can be used as an effective determination method. |
| Keywords: | Crataegus pinnatifida leaf Liquid chromatography Vitexin Hyperin Rutin |
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