| 赤芍总苷提取与大孔树脂纯化工艺研究 |
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| 引用本文： | 周洪伟,许文博,王玉蓉,韩振蕴.赤芍总苷提取与大孔树脂纯化工艺研究[J].中医药学报,2011,39(1):48-51. |
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| 作者姓名： | 周洪伟 许文博 王玉蓉 韩振蕴 |
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| 作者单位： | 1. 北京中医药大学中药学院,北京,100102
2. 北京中医药大学,东方医院,北京,100078 |
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| 基金项目： | 国家科技重大专项课题(编号:2009ZX09103) |
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| 摘 要： | 目的：优化赤芍总苷的提取与大孔树脂纯化工艺。方法：以芍药苷含量为指标,采用正交试验方法探讨乙醇浓度、乙醇用量和提取时间对提取结果的影响;考察大孔树脂型号、吸附流速、树脂径高比、水洗除杂体积、洗脱溶剂浓度、洗脱溶剂体积和上样浓度对纯化结果的影响。结果：选取工艺为12或10倍量（BV）80%乙醇提取,每次1.0h,提取2次,提取液过AB-8型大孔吸附树脂柱（树脂径高比为1∶8）,吸附流速1mL.m in^-1,3BV蒸馏水洗涤,5BV20%乙醇洗脱。结论：该工艺简单经济,可用于赤芍的提取与纯化。
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| 关 键 词： | 赤芍 提取 纯化 AB-8型大孔树脂 |
A Research on the Optimized Extraction Process and Macroporous Resin Purification Technology of Radix Paeoniae Rubra |
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| ZHOU Hong-wei,XU Wen-bo,WANG Yu-rong,HAN Zhen-yun.A Research on the Optimized Extraction Process and Macroporous Resin Purification Technology of Radix Paeoniae Rubra[J].Acta Chinese Medicine and Pharmacology,2011,39(1):48-51. |
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| Authors: | ZHOU Hong-wei XU Wen-bo WANG Yu-rong HAN Zhen-yun |
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| Institution: | ZHOU Hong-wei1,XU Wen-bo1,WANG Yu-rong1,HAN Zhen-yun2(1.School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 100102,China,2.Beijing University of Traditional Chinese Medicine subsidiary Dongfang Hospital,Beijing 100078,China) |
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| Abstract: | Objective: To study the optimized extraction process and macroporous resin purification technology of radix paeoniae rubra.Methods: Taking paeoniflorin as the characteristic markers,such as alcohol concentration,alcohol consumption and extraction time,which were the three factors.An orthogonal design was employed.Then selected the type of resin and investigated the adsorption velocity,resin diameter length ratio,volume of water and elution,concentration of elution and sample on the purification results.Resu... |
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| Keywords: | Radix paeoniae rubra Extraction Purification AB-8 macroporous resin |
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