芦荟大黄素抑制人口腔癌KB细胞增殖和迁移的双重效应及其机制研究

目的 探讨芦荟大黄素抑制人口腔癌细胞生长和迁移的双重效应及其机制.方法 对人口腔上皮癌KB细胞分别用2.5、5、10、20和40 μmol/L的芦荟大黄素处理,对照组为含0.1%二甲基亚砜的培养液,分别用结晶紫实验和划痕损伤愈合迁移实验分析长效抗增殖作用和抗迁移效应,并用Western blotting方法 检测蛋白激酶Ca和c-myc蛋白水平的变化.结果 芦荟大黄素能长时间抑制KB细胞的生长;划痕损伤愈合迁移实验结果 显示,药物处理3 d后进入划痕区域的细胞数为(6.2±0.2)个/mm2,而对照组为(33.5±0.4)个/mm2,差异有统计学意义(P＜0.05);对照组细胞抑制蛋白激酶Ca和c-myc的水平较高,经芦荟大黄素处理后,均以剂量依赖方式下降.结论 芦荟大黄素的抗增殖和抗迁移活性与抑制蛋白激酶C信号传导途径有关.

The anti-proliferation and anti-migration dual effects of aloe-emodin on KB cells and its mechanism

Objective To investigate the anti-proliferation and anti-migration dual effects of aloeemodin on KB cells and its mechanisms.Methods KB cells were treated with 2.5,5,10,20 and 40 μmol/L aloe-emodin.Crystal violet assay was used to determine the long-term growth inhibition of aloeemodin on human oral cancer KB cells.Scratch wound-healing motility assay was used to measure the antimigration effect.The protein kinase Cα and c-myc expression changes in protein levels were detected by Western blotting.Results A durable cell growth inhibitory effect of aloe-emodin on KB cells was found.Treatment of aloe-emodin resulted in the inhibition of cell migration.The protein kinase Cα and c-myc in protein levels were decreased upon treatment with aloe-emodin compared with controls.Condusions The anti-proliferation and anti-migration effects of aloe-emodin on KB cells are associated with the suppression of protein kinase C pathway.