人巨噬细胞金属弹性蛋白酶在胃癌细胞株及胃癌组织中的表达及意义

目的测定人巨噬细胞金属弹性蛋白酶（HME）在胃癌细胞及胃癌组织中的表达,评价其在胃癌进展中的作用.方法2003年4月至8月胃肠外科和肿瘤外科胃癌住院手术病人共58例,所有病人均取肿瘤组织、癌旁组织、远处正常组织标本,采用R-PCR、实时荧光定量PCR测定胃癌细胞株（MGC-803、SGC-7901、AGS）及胃癌组织中HME mRNA水平,采用Western印迹和免疫组化法测定HME蛋白水平.结果3株胃癌细胞株中均有HME mRNA和蛋白表达;胃癌组织中的HMEmRNA和蛋白表达高于远处正常组织,差异有统计学意义（P＜0.05）;而胃癌组织与癌旁组织中的HME mRNA和蛋白表达差异无统计学意义（P＞0.05）.结论胃癌患者中HME mRNA和蛋白呈一定比例的阳性表达,胃癌组织中最高,其次为癌旁组织和远处正常组织,说明HME是一项有潜在价值的肿瘤相关标志物.

The expression and significance of human macrophage metalloelastase in both gastric cancer cell lines and gastric cancer tissues

Objective To investigate the expression of human macrophage metalloelastase(HME) both in gastric cancer cell lines and gastric cancer tissues,and to find the role of HME in gastric carcino genesis.Methods Fifty eight patients who were operated in our hospital during April to Aug.2003 were enrolled.The samples taken from cancer,paracancer or normal tissues of these patients and cancer cell lines(MGC-803,SGC-7901,AGS)were detected for HME protein and HME mRNA expressions by Western blot and immunohistochemistry,RT-PCR and fluorescence quantitative PCR,respectively. Results Both HME mRNA and HME protein expressions were found in all three gastric cancer cell lines.The expressions of HME mRNA and HME protein in gastric cancer tissues was increased signifi- cantly compared with that in normal tissues(P<0.05).However,there was no significant difference in HME mRNA and HME protein expressions between gastric cancer tissues and paracancer tissues (P>0.05).Conclusions The increased HME expression in gastric cancer tissures compared with normal tissue indicate that HME may be a potential tumor marker for gastric cancer.