3种牙科金属材料对小鼠成纤维细胞凋亡的影响

背景:有关磁性附着体外包裹不锈钢材料引起的细胞凋亡,经检索CNKI数据库未见报道.目的:观察磁性附着体的3种外包裹牙科金属材料钴铬合金、钛合金及奥氏体不锈钢对L-929细胞凋亡的影响.设计、时间及地点:对比观察,实验于2007-06/12在中国医科大学中心实验室完成.材料:选用对数生长期的小鼠成纤维细胞L-g29,由中国医科大学附属第一医院肿瘤研究所提供.钴铬合金由贺立氏古莎齿科有限公司提供:钛合会由日本森田公司提供;奥氏体不锈钢由中国科学院金属研究所提供.方法:将钛合金、奥氏体不锈钢和钴铬合金3种金属材料制成圆片形,放置于24孔板中,按浸提液体积与试件表面积比值为0.1 mL/cm2放入RPMI-1640培养液,即得到材料浸提液.实验分为5组:钛合金组、奥氏体不锈钢组和钴铬合金组分别将L-929细胞接种于含不同质量浓度(250,500 g/L,1 kg/L)钛金属、奥氏体不锈钢和钴铬金属的浸提液中;阴性对照组:RPMI1640培养的L-929细胞:阳性对照组:1 00 mg/L丝列霉素处理的L-929细胞.主要观察指标:流式细胞仪检测各组不同质量浓度浸提液干预24 h对L-929细胞凋亡的影响.结果:除250 g/L质量浓度钛合金与阴性对照组之间细胞凋亡率无显著差异(P>0.05)外,其他各组材料同一质量浓度或同一材料不同质量浓度之间细胞凋亡率相比,差异均有显著件意义(P<0.01).结论:3种金属材料对细胞凋亡率均有显著影响,凋亡率大小依次为:钛合金组<奥氏体不锈钢组<钴铬合金组

Effects of three dental metal materials on mouse fibroblast apoptosis

BACKGROUND: There have been no reports in CNKI database addressing the cellular apoptosis caused by stainless steel materials used in the dental magnetic attachment. OBJECTIVE: To observe the effects of 3 stainless steel materials used in the dental magnetic attachment, cobalt-chromium alloy, titanium alloy, and austenitic stainless steel on L-929 cell apoptosis. DESIGN, TIME AND SETTING: A comparative observation was performed at the Central Laboratory of China Medical University between June and December 2007. MATERIALS: Mouse L-929 fibroblasts in logarithmic growth phase were provided by Institute of Tumor, First Affiliated Hospital of China Medical University, China. Cobalt-chromium (Co-Cr) alloy was purchased from Heraeus-Kulzer Corporation, China. Titanium alloy was obtained from Morita Company, Japan. Austenitio stainless steel was provided by Institute of Mental Research Chinese Academy of Sciences, China. METHODS: The leaching liquor of Co-Cr alloy, titanium alloy, and austenitic stainless steel was made by culturing 3 kinds of round-slice mental materials in 24-well plate with RPMI-1640 medium at 0.1 mL/cm2 (leaching liquor volume: specimen surface area). L-929 fibroblasts were divided into 5 groups: Co-Cr alloy, titanium alloy, austenitic stainless steel, negative control, and positive control. The Co-Cr alloy, titanium alloy, and austenitic stainless steel groups were cultured with corresponding leaching liquor (250, 500, and 1 000 g/L). Cells from the negative control and positive control groups were cultured with simple RPMI 1640 medium and 100 mg/L mitomycin-treated L-929 cells. MAIN OUTCOME MEASURES: Following 24-hour culture, the effects of 3 kinds of leaching liquor (at different concentrations) on L-929 fibroblasts were examined through the use of flow cytometer. RESULTS: There was significant difference in cellular apoptosis between Co-Cr alloy, titanium alloy, and austenitic stainless steel groups at the same concentration or between different concentrations for the same dental material (P < 0.01), but no significant difference existed between titanium-alloy (250 g/L) and negative control groups (P > 0.05). CONCLUSION: Co-Cr alloy, titanium alloy, and austenitic stainless steel have apparent influence on cellular apoptosis. The apoptosis rate is the greatest in the Co-Cr alloy group, followed by austenitic stainless steel group, and lastly titanium alloy group.