大鼠胚胎神经干细胞黑质内移植后的存活及分化

目的：观察胚胎神经于细胞脑内移植后的存活及生长分化状况。方法：从孕11d(E11d)大鼠胚胎神经管获取神经上皮细胞，经神经巢蛋白(nestin)染色鉴定干细胞；同时植入同种大鼠黑质内。于移植后7d、14d取脑，用神经元特异烯醇化酶(NSE)、酪氨酸羟化酶(TH)免疫组化方法检测移植细胞的存活及分化状况。结果：E11d神经管上皮细胞多数呈nestin染色阳性，黑质内移植后增殖形成细胞团并随时间延长而增大。免疫组化染色显示移植细胞团内有NSE及TH免疫阳性细胞。结论：胚胎神经上皮细胞多数为神经干细胞，黑质内移植后可以存活并分化为多巴胺能神经元。

Survival and differentiation of embryonic neural stem cells following transplantation into substantia nigra in rats

Objective : To observe the survival and differentiation of embryonic neuroepithelial cells in substantia nigra after transplantation. Methods : Neuroepithelial cells were dissociated from neural tube of embryonic Wistar rats (E11d), and the expression of nestin was examined, then they were transplanted into the substantia nigra of homogenous rats. The hosts were anesthetized and transcardially perfused 7d and 14d after transplantation respectively. Coronal sections of the brain were cut with in cryostat microtome. Using immunocvtochemistry the survival of neuroepithelial cells transplant, expression of neuro-specific enolase (NSE) and tyrosine tiydroxylase(TH) were examined. Results: Most neuroepithelial cells were nestin-positive. By immunocytochemistry, a mass of NSE-positive cells and TH-positive cells could be detected in the core of graft region. Conclusion: Present findings demonstrate that embryonic neural stem cells dissociated from neural tube can survive and differentiate into dopamine neurons.