脂多糖对人脐静脉内皮细胞ET-1、eNOS、iNOS mRNA表达的影响

目的：观察脂多糖（LPS）对人脐静脉内皮细胞ET-1、eNOS、iNOSmRNA表达的影响，在分子水平上进一步探讨LPS影响人脐静脉内皮细胞分泌ET-1、NO的机制。方法：选用体外培养的第3代人脐静脉内皮细胞，以100 μg/L浓度的LPS与之共孵育6 h。提取总RNA，运用半定量RT-RCR的方法，对ET-1、eNOS、iNOS mRNA的表达情况进行分析。结果：ET-1的灰度比值分别为：正常对照组0.82，LPS组1.32；eNOS的灰度比值分别为：正常对照组1.20，LPS组0.65；iNOS组的灰度比值分别为：正常对照组0.20，LPS组0.19。结论：低浓度的LPS对人脐静脉内皮细胞ET-1 mRNA的表达有促进作用，对eNOS mRNA的表达有抑制作用，对iNOS mRNA的表达则无显著作用。

Effects of LPS on expressions of ET-1, eNOS and iNOS mRNA in human umbilical vein endothelial cells

AIM: To observe the direct effect of LPS on expressions of ET-1, eNOS, and iNOS mRNA in human umbilical vein endothelial cells, and further research the molecular mechanism of effect of LPS on production of ET-1 and NO. METHODS:The third passage of cultured human umbilical vein endothelial cells was incubated with low concentration (100 μg/L) of LPS for 6 h. Total RNA was extracted. The expressions of ET-1, eNOS, and iNOS mRNA were analyzed by semi-quantitative RT-PCR method. RESULTS: ET-1 mRNA experession increased significantly, while expression of eNOS mRNA decreased significantly, and there was no significant change in expression of iNOS mRNA. CONCLUSION: In human umbilical vein endothelial cells, low concentration of LPS enhanced the expression of ET-1 mRNA, inhibited the expression of eNOS mRNA, and had no significant effect on the expression of iNOS mRNA.