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As_2O_3诱导胃癌细胞凋亡时蛋白激酶C及cAMP水平的变化
引用本文:李勇,刘冀红,赵群,马志学,刘品一,于跃明,范立侨. As_2O_3诱导胃癌细胞凋亡时蛋白激酶C及cAMP水平的变化[J]. 河北医科大学学报, 2001, 0(4)
作者姓名:李勇  刘冀红  赵群  马志学  刘品一  于跃明  范立侨
作者单位:河北医科大学第四医院外科!石家庄050011(李勇,刘冀红,赵群,马志学,刘品一,于跃明),河北医科大学第四医院外科!石(范立侨)
摘    要:目的从第二信使角度探讨As2 O3 诱导胃癌BGC 82 3细胞凋亡的作用机制。方法采用免疫组化、流式细胞术 ,观察As2 O3 诱导胃癌细胞凋亡的形态学改变及凋亡率 ;采用放免方法检测As2 O3 诱导胃癌细胞凋亡时环磷酸腺苷、蛋白激酶C水平变化。结果As2 O3 能够显著增加细胞凋亡率 (P <0 .0 5 ) ,诱导细胞出现凋亡形态学改变 ;并上调胃癌细胞内环磷酸腺苷水平 (P <0 .0 1) ,下调蛋白激酶C的水平 (P <0 .0 5 )。结论As2 O3 通过上调环磷酸腺苷水平 ,下调蛋白激酶C水平诱导胃癌细胞凋亡 ,抑制细胞增殖。

关 键 词:胃肿瘤  细胞衰老  砷剂  蛋白激酶C

CHANGES OF cAMP AND PKC IN As_2O_3 INDUCED APOPTOSIS IN GASTIC CELLS
Li Yong Liu Jihong Zhao Qun Ma Zhixue Liu Pinyi Yu Yueming Fan Liqiao. CHANGES OF cAMP AND PKC IN As_2O_3 INDUCED APOPTOSIS IN GASTIC CELLS[J]. Journal of Hebei Medical University, 2001, 0(4)
Authors:Li Yong Liu Jihong Zhao Qun Ma Zhixue Liu Pinyi Yu Yueming Fan Liqiao
Affiliation:Shijiazhuang 050011
Abstract:ObjectiveTo illustrate changes of cAMP and protein kinase C(PKC) in arsenic trioxid (As 2O 3) induced apoptosis in gastric cancer cells.MethodsCellular morphologic changes of BGC 823 cells was observed with immunohistochemistric way. Apoptosis rate was detected by flow cytometry. Radioimmunoassay was used to detect cAMP and PKC. ResultsApoptosis rate induced by As 2O 3 was higher than control (P< 0.05 ). The level of cAMP increased (P< 0.01 ), and that of PKC decreased (P< 0.05 ) in As_2O_3-treated cells. Gastric cancer cells presented morphologic features of apoptosis.ConclusionAs_2O_3 induced BGC-823 cells apoptosis by up-reglating cAMP and down-regulating PKC.
Keywords:stomach neoplasms  cell aging  arsenicals  protein kinase C
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