卵巢癌细胞中RNF123对p27Kip1蛋白稳定性的调控作用

目的:研究卵巢癌细胞中RNF123对p27Kip1蛋白稳定性的调控作用?方法:流式细胞分析仪测定血清饥饿释放过程中SKOV3细胞的周期分布情况,利用Western blot检测该过程中干扰RNF123前后p27Kip1蛋白的表达水平?在SKOV3细胞中转染sictrl和siRNF123,Western blot检测p27Kip1的半衰期?结果:SKOV3细胞血清饥饿48 h,SKOV3细胞周期阻滞在G1/G0期,而血清释放后S期显著增加?在此过程中,p27Kip1表达下调?转染siRNF123组相较于sictrl组,p27Kip1蛋白水平增高?降低RNF123表达后,p27Kip1的半衰期延迟?结论:在卵巢癌细胞中降低RNF123的表达能抑制p27Kip1的降解?

The role of RNF123 in regulating protein stability of p27Kip1 in epithelial ovarian cancer cells

Objective:To study the role of RNF123 in regulating protein stability of p27Kip1. Methods: Distribution of cell cycle was detected the by flow cytometer in SKOV3 cells,after treatment with serum starvation and release for synchronization purpose. Western blot was used to test the protein level of p27Kip1 of both sictrl cells and siRNF123 cells. SKOV3 cells were transfected with sictrl and siRNF123,western blot was used to analyze half-life of p27Kip1. Results:After serum deprivation for 48 h,SKOV3 cells were arrested in the G1/G0 phase,then serum releasing stimulated the proliferation of G1/G0 to S phase,and the expression of p27Kip1 was decreased during the progress. Moreover,the level of p27Kip1 was higher in siRNF123 cells than that in sictrl cells. After deceasing p27Kip1 expression,half-life of p27Kip1 was delayed. Conclusion:Our results suggest that down-regulated RNF123 can inhibit the degradation of p27Kip1.