门静脉主干缩窄附加脾静脉结扎制备犬门脉高压症模型

目的 观察犬门静脉主干缩窄加脾静脉结扎后门静脉系统的变化,为该方法能否建立一种具有脾亢的门静脉高压症大动物模型提供依据.方法 犬15条,采用门静脉主干缩窄加脾静脉结扎的方法建立模型,术前及术后每周观察实验动物血象的变化,在预定时间点分别随机选择5条犬开腹观测门静脉自由压、脾脏大小、门体侧支循环形成情况,并切取脾脏行组织病理学检查.此外,术前及术后预定时间点了解骨髓增生情况变化.结果 建模后,外周血红细胞、血小板下降,脾脏肿大明显,并且能够有效持续到第9周;术后第5、7、9周的脾脏组织病理学改变符合脾脏慢性淤血改变,而且骨髓增生情况较正常明显活跃.结论 门静脉主干缩窄加脾静脉结扎后门静脉系统的变化符合门静脉高压症的表现,尤其是脾亢状态较满意,具有良好的实验应用价值.

Establishment of a model of portal hypertension by portal vein coarctation and splenic vein ligation in dogs

Objective To observe the changes of portal vein system after coarctation of portal vein and ligation of splenic vein with the purpose of making sure whether it could prepare a model of portal hypertension with hypersplenism or not.Methods The model was established through coarctation of portal vein and ligation of splenic vein in 15 dogs.Monitoring the hemogram preoperative and weekly after the op-eration.At the point of the 5th,Tth,9th week after the operation, respectively and randomly selected 5 ani-mals to observe the condition of portal vein pressure, sizes of spleen and the collateral circulation, in addi-tion, also to take the pathology examination of the spleens and detect the accrementition of bone marrow.Results After coarctation of portal vein and ligation of splenic vein, the RBC and PLT counts decreased obviously, and could effectively last for 9 weeks.The splenic histopathology changes conformed to the chan-ges of the congestive splenomegaly, and the accrementition of bone marrow was much more active.Conclu-sion The alleosis of portal vein system conformed to the Portal hypertension,especially the condition of hypersplenism was satisfactory, so the model would have favourable value of scientific research.