| Effect of basic fibroblast growth factor on the expression of glial fibrillary acidic protein after tractive spinal cord injury in rats |
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| 引用本文： | 刘雷,吕波,屠重棋,池雷霆,王光林,裴福兴. Effect of basic fibroblast growth factor on the expression of glial fibrillary acidic protein after tractive spinal cord injury in rats[J]. 中华创伤杂志(英文版), 2005, 8(2): 117-120 |
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| 作者姓名： | 刘雷 吕波 屠重棋 池雷霆 王光林 裴福兴 |
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| 作者单位： | Department of Orthopaedic Surgery，West China Hospital of Sichuan University，Chengdu 610041，China，Department of Orthopaedic Surgery，West China Hospital of Sichuan University，Chengdu 610041，China，Department of Orthopaedic Surgery，West China Hospital of Sichuan University，Chengdu 610041，China，Department of Orthopaedic Surgery，West China Hospital of Sichuan University，Chengdu 610041，China，Department of Orthopaedic Surgery，West China Hospital of Sichuan University，Chengdu 610041，China，Department of Orthopaedic Surgery，West China Hospital of Sichuan University，Chengdu 610041，China |
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| 摘 要： | Basicfibroblastgrowthfactor(bFGF) isanewneurotrophicfactor(NTF)andhasbeencloselystudiedinrecentyears.1 Itsneurotrophiceffectsincludepromotioninneuronalregenerationandsurvival. StudieshavefoundthatexogenousbFGFpumpedintothemarroworthesheathcandecreasetissu…
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| 关 键 词： | 基础纤维原细胞生长因子 神经胶质 酸化蛋白质 脊椎损伤 基因表达 |
Effect of basic fibroblast growth factor on the expression of glial fibrillary acidic protein after tractive spinal cord injury in rats |
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| LIU Lei,U Bo,TU Chong-qi,CHI Lei-ting,WANG Guang-lin,PEI Fu-xing. Effect of basic fibroblast growth factor on the expression of glial fibrillary acidic protein after tractive spinal cord injury in rats[J]. Chinese journal of traumatology, 2005, 8(2): 117-120 |
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| Authors: | LIU Lei U Bo TU Chong-qi CHI Lei-ting WANG Guang-lin PEI Fu-xing |
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| Affiliation: | Department of Orthopaedic Surgery, West China Hospital of Sichuan University, Chengdu 610041, China |
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| Abstract: | OBJECTIVE: To investigate the effects of basic fibroblast growth factor (bFGF) on the expression of glial fibrillary acidic protein (GFAP) after tractive spinal cord injury in rats and to explore the recovery of spinal cord function. METHODS: The rats were subjected to tractive spinal cord injury at T13-L2. Cortical somatosensory-evoked potential (CSEP) was closely monitored and when P1-N1 wave amplitude decreased to 70% of that before operation, a small-bore catheter was inserted below the injured plane through subarachnoid cavity. In the treatment groups, 20 microl of bFGF solution (containing 20 microg of bFGF) was injected through the catheter right after the operation and 1, 2, 3, 4, 8, 12 and 24 h postoperatively. In the control group, same volume of normal saline was injected and every four rats were killed at 1, 4, 7, 14 and 21 d after the operation. Combined behavior score (CBS) and electro-physiological examination were adopted to evaluate function recovery. Expression of GFAP was observed by immuno-histochemical staining and was analyzed quantitatively by computer image analysis. RESULTS: There was statistically significant difference in GFAP-positive cells between bFGF treatment group and the control group (P<0.01). Similar tendency was indicated by the results of CBS and CSEP. CONCLUSIONS: bFGF can induce large expression of GFAP after tractive spinal cord injury in rats and promote spinal function recovery, which is highly important for spinal cord regeneration. |
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| Keywords: | Spinal injuries Basic fibroblast growth factor Glial fibrillary acidic protein Gene expression traction |
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