| 广西不同产地华南忍冬中绿原酸含量测定 |
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| 引用本文： | 黄陆良,丰杰,辛宁.广西不同产地华南忍冬中绿原酸含量测定[J].中国中医药信息杂志,2009,16(1):58-59. |
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| 作者姓名： | 黄陆良 丰杰 辛宁 |
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| 作者单位： | 广西中医学院,广西,南宁,530001 |
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| 基金项目： | 广西科技厅科技攻关项目，广西高校人才小高地建设创新团队资助计划 |
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| 摘 要： | 目的测定广西不同产地华南忍冬中绿原酸含量，评价华南忍冬药材质量。方法采用高效液相色谱法，Angilent ODS C18（4.6mm×150mm，5μm）柱，以乙腈-甲醇-0．05％磷酸溶液为流动相，梯度洗脱，三相的比例0～20min为7：5：88，25min为13：13：74；流速为0．5mL／min；检测波长为327nm；柱温为30℃。结果广西不同产地华南忍冬中绿原酸含量范围为2．21％～3．94％。结论广西不同产地华南忍冬中绿原酸含量存在一定的差异，但均已达到《中华人民共和国药典》的要求。
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| 关 键 词： | 华南忍冬 绿原酸 高效液相色谱法 含量测定 |
Content Determination of Chlorogenic Acid in Fructus Lonicera Confusa DC from Different Places in Guangxi by HPLC |
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| HUANG Lu-liang,FENG Jie,XIN Ning.Content Determination of Chlorogenic Acid in Fructus Lonicera Confusa DC from Different Places in Guangxi by HPLC[J].Chinese Journal of Information on Traditional Chinese Medicine,2009,16(1):58-59. |
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| Authors: | HUANG Lu-liang FENG Jie XIN Ning |
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| Institution: | (Guangxi College of TCM, Nanning 530001, China) |
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| Abstract: | Objective To determine the content of chlorogenic acid in Fructus Lonieera Confusa DC （FLCD） from different places in Guangxi by HPLC, and evaluate its intemal quality. Methods HPLC separation was performed on a Agilent C18 analytical column （4.6mm×150mm,5μm） by gradient eluting with acetonitrile-methanol-0.05% phosphoric acid at the flow rate of 0.5 mL/min. The column temperature was 30 ℃ and the UV detection wavelength was 327 nm. Results The chlorogenic acid content of FLCD from different places in Guangxi ranged from 2.21% to 3.94%. Conclusion The chlorogenic acid contents of FLCD from different places in Guangxi are different, but all of them are up to the standard of Phamarcopoeia of the People＇s Republic of China. |
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| Keywords: | Fructus Lonicera Confusa DC chlorogenic acid HPLC content determination |
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