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Sperm immobilized before intracytoplasmic sperm injection undergo ultrastructural damage and acrosomal disruption |
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| Authors: | Gómez-Torres Maria José Ten Jorge Girela José L Romero Jesús Bernabeu Rafael De Juan Joaquin |
| Affiliation: | a Department of Biotechnology, University of Alicante, Alicante b Instituto Bernabeu of Fertility and Gynaecology, Alicante, Spain c Department of Reproductive Medicine, Miguel Hernandez University, Elche, Spain |
| Abstract: | The aim of this study was to describe morphologic sperm changes observed via scanning electron microscopy and the acrosomal status resulting from immobilization and micromanipulation. The manipulations made on sperm before intracytoplasmic sperm injection were the possible cause of sperm ultrastructural damages and could trigger an acrosome reaction. |
| Keywords: | Acrosome reaction human spermatozoa ICSI scanning electron microscopy |
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