人乳头瘤病毒16型E＿6E＿7基因反义质粒对人宫颈癌细胞恶性的逆转作用

构建可为地塞米松诱导表达的人乳头瘤病毒１６型（ＨＰＶ－１６）Ｅ＿６Ｅ＿７基因反义质粒（ｐ１６ａｓＥ＿６Ｅ＿７Ｎｅｏ），利用磷酸钙沉淀法将其分别转染到ＨＰＶ－１６阳性的人宫颈癌细胞株Ｃａｓｋｉ和ＨＰＶ阴性的人宫颈癌细胞株Ｃ－３３Ａ中。地塞米松诱导反义质粒表达后，ＣａｓＫｉ细胞失去其恶性表型，而Ｃ－３３Ａ细胞的生长特性及恶性行为未发生变化。说明反义质粒能够改变Ｃａｓｋｉ细胞的恶性表型，且这种改变是通过特异性抑制Ｅ＿６Ｅ＿７基因表达实现的。

The reversing effect of HPV-16 E_6E_7 gene antisense plasmid on the HPV-16 positive human cervical carcinoma cells

ntisense reconstruct,p16as E6E7Neo, harboring HPVI6E6E7 gene of which the expression is regulatable by dexamethasone was constructed.Calcium phosphate mediated transfection was employed to transduce this antisense reconstruct into HPV-16 positive CasKi cell line and HPV negative C-33A cell line(both derived from human cervical carcinoma)respectively. After dexamethasone was added in-to the culture medium to induce the expression of E6E7 antisense gene, the malignity of CasKi cells were reversed,while the growth characteristics and malignity of C- 33A cells were unchanged.The results show that the reversing effect on CasKi cell is specific and mediated by inhibition of expression of E6E7 gene, It also demonstrated a more profound pathogenic role of HPV-16 E6E7 gene in the tumorogenesis of cervical carcinoma and provides a possibility for gene therapy of this tumor.