电针遏制癫痫小发作时脑内氨基酸含量的变化

目的 :本课题前阶段已证明电针能遏制癫痫小发作 ,现拟探查此时脑内一些氨基酸含量的变化。方法 :在戊四氮注射及闪光刺激造成癫痫小发作的大鼠 ,收集脑内一些部位在电针穴位前后的灌流液 ,测定四种氨基酸的含量。结果 :皮层运动区、视区的γ 氨基丁酸、甘氨酸及牛磺酸在电针后显著增加 ,谷氨酸减少。丘脑网状核、黑质网状部的γ 氨基丁酸亦明显增加。结论 :电针促使脑内与抑制有关的氨基酸含量增加 ,而兴奋性氨基酸有减少趋势

Effect of Electroacupuncture on Brain Amino Acid Contents during Epileptic Petit Mai in the Rat

Objective: To observe changes of amino acid contents in the brain during electroacupuncture (EA) induced suppression of epileptic petit mal. Methods: A total of 5 SD rats with epilepsy were observed in the present paper. Epileptic petit mal was produced by intraperitoneal injection of pentylenetetrazol (PTZ, 35 mg/kg) or PTZ (15 mg/kg) injection plus neon-lamp light stimulation (0.5 Hz, 60 sec) of the eye. "Dazhui"(GV 14) and "Jizhong"(GV 6) were punctured and stimulated electrically for 20 min with G6805 EA Instrument by setting the stimulating parameters as electric current of 5 mA and frequency of 80 Hz. Perfusion fluid samples were collected from the sensory-motor area and visual area of the cerebral cortex, reticular nucleus of the thalamus and reticular part of the substantia nigra via the implanted cannula for detecting glutamate (Glu), γ-aminobrtyric acid (GABA), glycine (Gly) and taurine (Tau) with high pressure liquid chromatography technique. Results: After acupuncture treatment, Glu (excitatory amino acid) contents of the sensory-motor area and visual area of the cerebral cortex decreased slightly but had no significant changes in comparison with pre-treatment. GABA (inhibitory amino acid) contents of the sensory-motor area and visual area, reticular nucleus of the thalamus and reticular part of the substantia nigra, Gly (inhibitory amino acid) contents of the sensory-motor area and visual area all increased considerably compared with pre-EA in rats with epileptic petit mal (P<0.05～0.01). Tau (the excitant of Gly receptor) contents of the sensory-motor area and visual area also increased remarkably (P<0.01). Conclusion: During epileptic petit mal, EA stimulation can lower the excitatory amino acid content to a certain degree and raise the inhibitory amino acid level of the brain significantly, checking epileptic petit mal of the rat