| ICU中MRSA耐药基因检测及其PFGE分型 |
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| 引用本文： | 庞杏林,李孝权,张颖,张欣强,胡玉山,邓志爱,陈守义,莫自耀. ICU中MRSA耐药基因检测及其PFGE分型[J]. 热带医学杂志, 2009, 9(3) |
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| 作者姓名： | 庞杏林 李孝权 张颖 张欣强 胡玉山 邓志爱 陈守义 莫自耀 |
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| 作者单位： | 1. 广州市疾病预防控制中心,广州,510080
2. 广州呼吸疾病研究所,广州,510230 |
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| 基金项目： | 广州市医药卫生科技项目(No.2006YB131)。 |
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| 摘 要： | 目的对耐甲氧西林金黄色葡萄球菌(MRSA)菌株进行分子分型,探讨ICU中MRSA医院感染的特点和流行规律。方法采用表型筛选和PCR扩增mecA基因方法鉴定MRSA菌株,脉冲场凝胶电泳方法(PFGE)进行分子分型。结果12株金黄色葡萄球菌表型筛选为MRSA,MRSA产生A型、B型、C型和D型4种耐药表型,优势耐药模式是A型(75.0%),MRSA对苯唑西林、阿莫西林/克拉维酸和氨苄西林/舒巴坦等10种抗生素产生100%耐药性,11株MRSA携带mecA基因,携带率为91.7%,PFGE指纹图谱分两型,分别为R1型和R2型,11株MRSA为R1型(91.7%),R1型各株间相似度为100%。结论ICU可存在MRSA爆发流行,MRSA产生多重耐药性(MDR),MRSA携带mecA基因可表现为MDR,PFGE分型是理想的分子流行病学溯源手段。
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| 关 键 词： | 耐甲氧西林金黄色葡萄球菌 流行病学 耐药基因 脉冲场凝胶电泳 |
The Analysis of MRSA Drug-Resistant Gene and PFGE from ICU |
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| PANG Xing-lin,LI Xiao-quan,ZHANG Ying,ZHANG Xin-qiang,HU Yu-shan,DENG Zhi-ai,CHEN Shou-yi,MO Zi-yao. The Analysis of MRSA Drug-Resistant Gene and PFGE from ICU[J]. Journal Of Tropical Medicine, 2009, 9(3) |
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| Authors: | PANG Xing-lin LI Xiao-quan ZHANG Ying ZHANG Xin-qiang HU Yu-shan DENG Zhi-ai CHEN Shou-yi MO Zi-yao |
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| Affiliation: | 1. Guangzhou Center for Diseases Prevention and Control;Guangzhou 510080;2. Guangzhou Institute of Respiratory Diseases;Guangzhou 510230;China |
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| Abstract: | Objective To investigate the feature and epidemiology of methicillin resistant Staphylococcus aureus (MRSA)-inducing nosocomial infection by molecule grouping for 12 strains of Staphylococcus aureus (SA) and analysis of clinical data. Methods To determine the MRSA strain by phenotype screening and PCR amplification of mecA gene, and molecule grouping for the MRSA strain by pulse field gel electrophoresis (PFGE). Results The phenotype screening display that all of the 12 strains of SA are MRSA. There are 4 t... |
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| Keywords: | MRSA epidemiology drug-resistance gene PFGE |
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