| rhG-CSF动员对异基因造血干细胞移植供者外周血CD4~+ T细胞S1P_1表达的影响 |
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| 引用本文： | 朱海燕,达万明,高春记,李猛,陈卫华,于力,黄文荣.rhG-CSF动员对异基因造血干细胞移植供者外周血CD4~+ T细胞S1P_1表达的影响[J].中国实验血液学杂志,2010,18(2):427-430. |
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| 作者姓名： | 朱海燕 达万明 高春记 李猛 陈卫华 于力 黄文荣 |
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| 作者单位： | 解放军总医院血液科,北京,100853 |
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| 基金项目： | 全军卫生科技攻关课题(十一五攻关课题)资助,编号06MA252 |
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| 摘 要： | CD4+ T细胞主要通过其表面的S1P1受体与外界的第一信使1磷酸鞘氨醇(S1P)相互作用调节免疫功能。本研究旨在探讨重组人粒细胞集落刺激因子(rhG-CSF)动员对异基因造血干细胞移植(allo-HSCT)供者外周血CD4+ T细胞S1P1表达的影响。17例allo-HSCT供者于rhG-CSF动员前及动员后第4天采集外周血,使用磁珠分选法纯化CD4+ T细胞,提取微量RNA,用实时定量PCR法检测S1P1的表达。结果显示,rhG-CSF动员前后CD4+ T细胞均表达S1P1,且动员后S1P1在CD4+ T细胞中的表达明显低于动员前。结论:rhG-CSF动员使allo-HSCT供者外周血CD4+ T细胞S1P1的表达明显下调。
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| 关 键 词： | 重组人粒细胞集落刺激因子 异基因造血干细胞移植 CD4＋T细胞 S1P1表达 |
Impact of rhG-CSF on Sphingosine 1-phosphase Receptor1 Expression in CD4+T Cells of Donor Peripheral Blood |
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| ZHU Hai- Yan,DA Wan-Ming,GAO Chun-Ji,LI Meng,CHEN Wei-Hua,Yü Li,HUANG Wen-Rong.Impact of rhG-CSF on Sphingosine 1-phosphase Receptor1 Expression in CD4+T Cells of Donor Peripheral Blood[J].Journal of Experimental Hematology,2010,18(2):427-430. |
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| Authors: | ZHU Hai- Yan DA Wan-Ming GAO Chun-Ji LI Meng CHEN Wei-Hua Yü Li HUANG Wen-Rong |
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| Institution: | (Department of Hematology, Chinese PLA General Hospital,Beijing 100853, China) |
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| Abstract: | CD4+ T cells mainly interact with Sphingosine 1-phosphase(S1P) to regulate immune function through Sphingosine 1-phosphase receptor 1(S1P1).This study was aimed to investigate the effects of recombinant human granulocyte-colony-stimulating factor(rhG-CSF) mobilization on S1P1 expression in CD4+ T cells of donor's peripheral blood.The CD4+T cells of peripheral blood were isolated by magnetic beads from 17 allo-hematopoietic stem cell transplantation(allo-HSCT) donors before and at fourth day of mobilization ... |
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| Keywords: | rhG-CSF allo-HSCT CD4+ T cell S1P1 expression |
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