减体积肝移植模型大鼠肝脏组织miRNAs表达谱变化 |
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作者姓名: | 高红强 李志强 王海富 薛国友 刘 静 陈 刚 李 立 |
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作者单位: | 昆明医科大学附属甘美医院、昆明市第一人民医院肝胆胰外科,云南省昆明市 650011 |
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基金项目: | 云南省应用基础研究计划-昆明医科大学联合专项重点项目(2012FB010) |
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摘 要: |
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关 键 词: | 实验动物 移植动物模型 大鼠减体积肝移植 消化系统损伤模型 肝脏 微小RNA miRNAs  microarray PCR 反转录 miRNAs表达谱 miRNAs芯片数据分析 |
收稿时间: | 2015-11-09 |
Changes in miRNAs expression profiling in the liver of a rat model after reduced-size liver transplantation |
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Authors: | Gao Hong-qiang Li Zhi-qiang Wang Hai-fu Xue Guo-you Liu Jing Chen Gang Li Li |
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Institution: | Department of Hepato-Biliary-Pancreatic Surgery, the First People’s Hospital of Kunming and the Ganmei Affiliated Hospital of Kunming Medical University, Kunming 650011, Yunnan Province, China |
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Abstract: | BACKGROUND: Liver regeneration is the key factor influencing the prognosis of living donor liver transplantation. There has not been the research on special miRNA of liver regeneration after living donor liver transplantation.
OBJECTIVE: To analyze the variation of miRNAs expression profile after rat reduced-size liver transplantation at certain time point, select and verify target miRNA which can provide targeting intervention strategies in liver regeneration after rat reduced-size liver transplantation and provide theoretical evidence for liver regeneration after living donor liver transplantation.
METHODS: The reduced-size liver transplantation models were established. miRNAs microarray was used to detect miRNA expression. In differentially expressed microRNAs, real-time quantitative PCR was utilized to detect target miRNAs. The credibility of miRNAs microarray results was verified.
RESULTS AND CONCLUSION: Compared with rat liver tissue in the sham operation group, 11 miRNAs up-regulated in reduced-size liver transplantation, including let-7b-5p, let-7c-5p, miR-101a-3p, miR-103-3p, miR-130a-3p, miR-142-5p, miR-186-5p, miR-199a-3p, miR-21-5p, 221-3p and miR-34a-5p. Four miRNAs were down-regulated, including miR-26b-5p, miR-150-5p, miR-19a-3p and rno-miR-146-5p. PCR test further verified that miR-221-3p and miR-199a-3p expression changes approximated the chip results at 24, 48 hours and 1 week, indicating that results of miRNA microarray were believable. These results verified that it exists variation of miRNAs expression profile after rat reduced-size liver transplantation, which picked out and verified the target miRNAs.
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