白芨胶载外源性重组人表皮生长因子促进伤口愈合机制

Mechanisms underlying the promotion of wound healing by bletilla carrying exogenous recombinant human epidermal growth factor

BACKGROUND: How to promote wound healing is always the research focus of the surgical physicians in the clinic. Recombinant human epidermal growth factor (rhEGF) can effectively promote wound healing. However, as a biological agent, it is easy to be decomposed under normal temperature. OBJECTIVE: To explore the mechanisms underlying the promotion of wound healing in the back of rabbits by bletilla carrying exogenous rhEGF. METHODS:Model rabbits with full-thickness skin defects in the back were treated with bletilla carrying exogenous rhEGF (combined treatment group), bletilla, rhEGF, or saline (control group). RESULTS AND CONCLUSION:The time of wound healing was the shortest (P < 0.05) and the wound healing rate was the highest (P < 0.05) in the combined treatment group. On postoperative days 3 and 10, newly formed granulation tissue, capillaries, and collagenous fibers showed by hematoxylin-eosin staining staining and Masson staining and the strongest immunoreactivity of vascular endothelial growth factor determined by immunohistochemical staining were found in the combined treatment group (P < 0.05). These findings confirm that bletilla carrying exogenous rhEGF promotes wound healing by accelerating the forming of granulation tissue, new-born capillaries, and collagenous fibers, and the effects are superior to either of them alone.