骨髓间充质干细胞促进肺癌转移的机制

Mechanism of bone marrow mesenchymal stem cells in promoting lung cancer metastasis

BACKGROUND:So far the positive or negative effects of mesenchymal stem cells on tumor growth and metastasis are under discussion. OBJECTIVE:To explore the mechanism of bone marrow mesenchymal cells in promoting lung cancer metastasis.METHODS: Primary rat bone marrow mesenchymal stem cells were obtained by direct adherence method of the whole bone marrow, and differential adherence combined with digestion control method was performed to purify cells. Lung cancer cell lines were cultured, and the effects of bone marrow mesenchymal stem cells on the migration, invasion and metastasis of lung cancer cells were observed by scratch test, cell invasion and migration assays. Orthotopic lung cancer models were established in rats and bone marrow mesenchymal stem cells were seeded onto the left lung of rats. Then, pathological changes of lung tissues were observed at 14 days after transplannation. RESULTS AND CONCLUSION:After the scratch test, the migration rate of lung cancer cells became higher, and the scratches healed with time. And after cell transplantation, the number of migrated lung cancer cells increased, as well as the ability of lung cancer cells penetrating the Matrigel was strengthened. Besides, fibrous connective tissues could be found around the lung cancer tissues, and necrosis with distinct boundary and large tumor nuclei; the metastatic tissues showed obvious infiltration and necrosis with large tumor nuclei. These results suggest that bone marrow mesenchymal stem cells can promote the invasion, migration and metastasis of lung cancer cell lines.