组织工程半月板：种子细胞和理化因素

BACKGROUND: As meniscectomy may result in various adverse reactions, tissue-engineered meniscus is expected to be used for meniscus repair. Seed cell selection and optimal physicochemical stimuli are crucial for the construction of tissue-engineered meniscus. OBJECTIVE:To overview the seed cells for the tissue-engineered meniscus construction and the research progress of physicochemical factors.METHODS: The first author retrieved the CNKI and Medline databases using the keywords of “meniscus, tissue engineering, seed cells, physical and chemical factors” in English and Chinese, respectively, to retrieve articles related to the seed cells and physicochemical factors of the tissue-engineered meniscus construction. Irrelative and repetitive articles were excluded, and 49 eligible articles were enrolled for analysis.RESULTS AND CONCLUSION:The seed cells must maintain their phenotype and synthetic ability after physicochemical stimulation in combination with scaffolds, to achieve the reproducibility of tissue-engineered meniscus. Most of cytokines can be used for promoting the proliferation and differentiation of chondrocytes, but the underlying mechanisms were little known. Their application in the meniscus tissue engineering needs to be studied in depth. Currently it is urgent to improve physicochemical stimuli in order to construct the tissue-engineered meniscus. The shear force does harm to chondrocyte phenotype, and dynamic compression loading has been proved to enhance Ca+ and glycosaminoglycan release. The fibrous cartilage stimulated by shear force and other factors may be helpful for constructing the tissue-engineered meniscus. An elaborated randomized controlled trial and the long-term quantitative analysis are of importance to assess the research results.中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Tissue-engineered meniscus: seed cells and physicochemical factors