骨髓间充质干细胞联合依达拉奉移植脑梗死大鼠血清炎性因子的表达

Expression of serum inflammatory cytokines in rats with cerebral infarction undergoing bone marrow mesenchymal stem cell transplantation combined with edaravone

BACKGROUND:To inhibit the expressions of prothrombin activator inhibitor 1 and tissue plasminogen activator is one potential target for the treatment of cerebral infarction.OBJECTIVE:To investigate the expressions of serum inflammatory cytokines in rats with cerebral infarction undergoing bone marrow mesenchymal stem cell transplantation combined with edaravone.METHODS:Sixty Sprague-Dawley rats were enrolled to prepare models of focal cerebral infarction by middle cerebral artery occlusion, and were randomly divided into four groups. Rats were given intravenous injection of PBS via tail veins for 5 consecutive days as model group, rats were subjected to intravenous injection of 2.0×10⁹ /L bone marrow mesenchymal stem cell suspension (1 mL) via tail veins, twice daily for 5 days as stem cell transplantation group, and those were given intravenous injection of 30 mg edaravone combined with intravenous injection of 2.0×10⁹/L bone marrow mesenchymal stem cell suspension (1 mL) via tail veins for 5 days, twice daily, as combined group.RESULTS AND CONCLUSION:Compared with the model group, modified neurologic severity scores were lower, expressions of serum prothrombin activator inhibitor 1 and tumor necrosis factor-α mRNA in the brain decreased, and the infarct area reduced in the stem cell transplantation and combined groups. And the changed levels of above indicators in the combined group were significantly larger than those of the stem cell transplantation group. In conclusion, combination of bone marrow mesenchymal stem cell transplantation with edaravone can promote neural function recovery after cerebral infarction.