复方地芬诺酯建立大鼠便秘模型及对肠道菌群的影响

BACKGROUND: In the previous studies, the overall index in constipation models, such as fecal output in 24 hours and intestinal tension are usually measured. However, the changes of intestinal flora are rarely involved. OBJECTIVE: To explore the effect of constipation on intestinal tension, pathology and microecology in the rat models of diphenoxylate-induced constipation. METHODS: Twenty SPF Wistar rats were randomly divided into blank control and model groups, followed by given 10 mg/kg diphenoxylate and distilled water by gavage once daily for 20 days, respectively. RESULTS AND CONCLUSION: Twenty days later, compared with the blank control group, the frequency of ileum contraction was decreased, 24-hour stool decreased, the ratio of bacteria group in the cecum changed, and structure of the colon was seriously damaged in the model group. These results suggest that constipation results in the intestinal flora disturbance remarkablely. 中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Effect of constipation induced by diphenoxylate on intestinal flora in a rat