CD133+细胞联合人脐血干细胞在心力衰竭小鼠中的作用

BACKGROUND: Currently, conventional treatment methods for heart failure are all ineffective.OBJECTIVE: To explore the combined effects of human umbilical cord stem cells and CD133+ cells in mice with heart failure, providing a new insight into the treatment of heart failure.METHODS: Full-term newborn umbilical cord from vaginal delivery was collected to isolate CD133+ cells and human umbilical cord stem cells using lymphocyte separation medium method. Twenty Balb/C nude mice were randomly subjected to mononuclear cell injection (mononuclear cell group) or injection of CD133+ cells combined with human umbilical cord stem cells (combined group) via the tail vein after establishing heart failure models. RESULTS AND CONCLUSION: Fourteen days after injection, the body weight and liver, heart and lung mass of mice were significantly larger in the combined group than the mononuclear cell group (P < 0.05). After 30 days, myocardial cells arranged regularly in the combined group, but disorderly in the mononuclear cell group; compared with the mononuclear cell group, the average area of myocardial collagen fibers was significantly decreased in the combined group (P < 0.05), and the level of serum matrix metalloproteinase-9 was also significantly lower in the combined group (P < 0.05). Masson staining showed that blue-stained collagen fibers in the combined group were less but arranged neatly; however, in the mononuclear cell group, the number of collagen fibers that arranged irregularly was increased to different extents. To conclude, the combined use of CD133+ cells and human umbilical cord stem cells has desired outcomes in the treatment of heart failure in mice, indicating a higher clinical value.中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

CD133+ cells in combination with human umbilical cord stem cells in mouse heart failure