左旋聚乳酸羟基磷灰石材料上人牙周膜细胞的生长

Growth of human periodontal ligament cells on the poly-L-lactic acid hydroxyapatite

BACKGROUND: In constructing tissue-engineered periodontium in vitto, the effective combination of seed cells with scaffold materials is the key to promote the quality of tissue-engineered periodontium, in which human periodontal ligament cells are commonly used.OBJECTIVE: To explore the growth of human periodontal ligament cells on the poly-L-lactic acid hydroxyapatite.METHODS: Human periodontal ligament cells were isolated and cultured in vitro, and passage 3 cells were chosen to be randomly divided into two groups: cells cultured alone as control group, and cultured with poly-L-lactic acid hydroxyapatite as experimental group. After 1, 2 and 3 days, alkaline phosphatase activity and expression of type III collagen in the two groups were detected, and the cell growth curve was depicted using MTT method.RESULTS AND CONCLUSION: By immunohistochemical staining, cultured cells were positive for vimentin and alkaline phosphatase staining and negative for anti-keratin staining, indicating that the cells were identified as human periodontal ligament cells. By MTT method, there was no significant difference in the absorbance value of two groups at different time points (P > 0.05). And after 1, 2 and 3-day co-culture, the alkaline phosphatase activity levels showed no significant difference between two groups (P > 0.05). Besides, no significant difference in the absorbance value of type III collagen was found in the two groups (P > 0.05). To conclude, the human periodontal ligament cells can grow and proliferate well on the poly-L-lactic acid hydroxyapatite scaffold with no cytotoxicity.