CD44+CD24-/low乳腺癌干细胞活性与多药耐药的相关性 |
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作者姓名: | 翟晓建 张 浩 张旖旎 倪 鸣 王 征 |
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作者单位: | 1南阳市中心医院乳腺甲状腺外科,河南省南阳市 473003
2南昌大学江西医学院,江西省南昌市 330000
3南阳市中心医院妇三科,河南省南阳市 473003 |
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摘 要: |
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关 键 词: | 干细胞 肿瘤干细胞 乳腺癌 细胞活性 细胞分选 多药耐药 体外成球实验 耐药基因 |
Relationship between cell activity and multidrug resistance of CD44+CD24-/low breast cancer stem cells |
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Authors: | Zhai Xiao-jian Zhang Hao Zhang Yi-ni Ni Ming Wang Zheng |
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Institution: | Department of Breast and Thyroid Surgery, Third Department of Gynecology, Nanyang Central Hospital, Nanyang 473003, Henan Province, China; Nanchang University Medical School, Nanchang 330000, Jiangxi Province, China |
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Abstract: | BACKGROUND:Tumor stem cells are found to be involved in the recurrence, metastasis and drug resistance of the tumor.
OBJECTIVE:To explore the relationship between cell activity and multidrug resistance of CD44+CD24-/low breast cancer stem cells.
METHODS: CD44+CD24-/low breast cancer stem cells sorted from multidrug resistant breast cancer cell line MCF-7/ADR were detected as percentage using flow cytometry. P-gp fluorescence intensity of the cell membrane and MDR mRNA expression in sorted cells and MCF-7/ADR were detected using flow cytometry and RT-PCR, respectively.
RESULTS AND CONCLUSION:After sorting by flow cytometry, the proportion of CD44+CD24-/low breast cancer stem cells was more than 90%, indicating that the sorted cells could meet the needs of the subsequent experiment. CD44+CD24-/low cell subsets exhibited stronger ability to form microspheres than non- CD44+CD24-/low cell subsets. The P-gp fluorescence intensity and MDR mRNA expression of CD44+CD24-/low cells were significantly higher than those of MFC-7/ADR cell line (P < 0.05). These experimental findings suggest that CD44+CD24-/low breast cancer stem cells sorted from MCF-7/ADR cell lines have a strong ability to form cell microspheres in vitro, and significantly raise the level of P-gp protein and MDR mRNA expression, which may be one of the causes of multidrug resistance. |
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Keywords: | Breast Neoplasms Neoplastic Stem Cells P-Glycoprotein Drug Resistance Neoplasm Antigens CD44 Antigens CD24 Tissue Engineering |
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