炎性细胞模型中姜黄素对胆固醇逆转运蛋白ABCA1和ABCG1基因的影响

目的 探讨炎性反应模型中,姜黄素对胆固醇逆转运蛋白基因表达的影响。 方法 将RAW264.7细胞分为6组:空白对照组,姜黄素10 μmol/L组,姜黄素20 μmol/L组,脂多糖(LPS)组,姜黄素10 μmol/L+LPS组和姜黄素20 μmol/L+LPS组。应用荧光定量PCR检测各组中RAW264.7细胞腺苷三磷酸结合盒转运体A1(ABCA1)、腺苷三磷酸结合盒转运体G1(ABCG1)和肿瘤坏死因子-α(TNF-α)基因表达情况。 结果 LPS刺激可显著上调RAW264.7细胞ABCA1和TNF-α基因的表达(P<0.05),下调ABCG1基因的表达(P<0.05);姜黄素处理后可进一步上调ABCA1基因的表达(P<0.05),但是对ABCG1和TNF-α基因的表达差异无统计学意义(P>0.05)。 结论 在炎性反应条件下,姜黄素可进一步上调胆固醇逆转运关键基因ABCA1的表达,这可能是其抗动脉硬化的分子机制之一。

Effects of curcumin on gene expressions of reverse cholesterol transport proteins ABCA1versus ABCG1 in the cell model of inflammation

Objective To explore the effects of curcumin on reverse cholesterol transport protein gene expression in the cell model of inflammation. Methods RAW264.7 cells were divided into 6 groups: control group, 10 μmol/L curcumin group, 20 μmol/L curcumin group, lipopolysaccharide(LPS)group, 10 μmol/L curcumin+LPS group, and 20 μmol/L curcumin+LPS group. The levels of mRNA expressions of ATP-binding cassette transporter A1(ABCA1), ATP-binding cassette transporter G1(ABCG1)and tumor necrosis factor-α(TNF-α)in each group were determined by Real-Time PCR. Results LPS induction significantly increased ABCA1 and TNF-α mRNA expressions, and decreased ABCG1 mRNA expression(all P<0.05). Pre-treating the cell with curcumin and stimulating with LPS did not change the expressions of TNF-α and ABCG1(both P>0.05), but significantly increased the ABCA1 mRNA expression(P<0.05). Conclusion Curcumin significantly amplifies the expression of reverse cholesterol transport key genes ABCA1 under the condition of the inflammatory reaction, which may be one of the molecular mechanisms of anti-arteriosclerosis.