PTEN基因沉默骨髓间充质干细胞移植治疗急性心肌梗死 |
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作者姓名: | 徐慧敏 刘玉茹 万琪琳 李彦明 吴昌维 |
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作者单位: | 河南大学淮河医院老年病科,河南省开封市 475000 |
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摘 要: |
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关 键 词: | 干细胞 移植 急性心肌梗死 第10号染色体同源丢失性磷酸酶张力蛋白基因 基因沉默 骨髓间充质干细胞 干细胞移植 |
收稿时间: | 2016-04-20 |
PTEN-silenced bone marrow mesenchymal stem cell transplantation for acute myocardial infarction |
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Authors: | Xu Hui-min Liu Yu-ru Wan Qi-lin Li Yan-ming Wu Chang-wei |
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Institution: | Department of Geriatrics, Huaihe Hospital of Henan University, Kaifeng 475000, Henan Province, China |
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Abstract: | BACKGROUND:Bone marrow mesenchymal stem cell transplantation for myocardial infarction becomes popularized in recent years, but transplanted cells cannot survive and proliferate under early inflammatory reaction or local ischemia/hypoxia microenvironment, eventually hampering the therapeutic outcomes.
OBJECTIVE:To investigate the therapeutic effect of PTEN-silenced bone marrow mesenchymal stem cells on acute myocardial infarction.
METHODS:(1) Bone marrow mesenchymal stem cells from Sprague-Dawley rats were randomly assigned to receive no treatment, NCsiRNA transfection using Lipofectamin2000 or PTEN siRNA transfection using Lipofectamin2000. Cell growth curves were described using MTT method to detect cell cycle using flow cytometry. (2) Thirty Sprague-Dawley rats were selected to prepare myocardial infarction models that were randomized into three groups (n=10 per group): blank control, negative control and RNAi group. Six hours after modeling, bone marrow mesenchymal stem cells transfected with nothing, NCsiRNA and PTEN siRNA were respectively injected into the infarcted center of the left ventricular anterior wall in these three rat groups. After 4 weeks, all rats were subjected to cardiac function detection using echocardiography, and the survival and proliferation of bone marrow mesenchymal stem cells in the rats were observed by fluorescence microscopy.
RESULTS AND CONCLUSION:Compared with the other two groups, a significant increase in the absorbance values at different culture time, the proportion of cells in S+G2 phase, and the number of bone marrow mesenchymal stem cells in the myocardial tissue was found in the RNAi group (all P < 0.05). Additionally, the left ventricular ejection fraction and left ventricular shortening fraction were significantly reduced in the RNAi group than the blank control and negative control groups at 4 weeks after cell transplantation (P < 0.05). Both in vivo and in vitro experimental findings showed that PTEN silencing could effectively improve cell survival and proliferation in the infarcted myocardium. Moreover, in the in vivo experiment, an overt improvement in rat’s cardiac function was achieved. |
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Keywords: | Myocardial Infarction PTEN Phosphohydrolase Bone Marrow Mesenchymal Stem Cells Gene Silencing Tissue Engineering |
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