脂肪间充质干细胞移植急性心肌梗死区域对心肌组织的保护作用

Adipose-derived mesenchymal stem cell transplantation protects the myocardial tissues from acute myocardial infarction

BACKGROUND: Adipose-derived mesenchymal stem cells have rich sources that are easily obtained, which can be used to treat acute myocardial infarction.OBJECTIVE: To investigate the therapeutic effect of adipose-derived mesenchymal stem cell transplantation on acute myocardial infarction in rats.METHODS: Rat models of acute myocardial infarction were made and subjected to adipose-derived mesenchymal stem cell transplantation in comparison with model and control (sham operation) groups.RESULTS AND CONCLUSION: Echocardiography findings showed significant improvement in the left ventricular end-systolic diameter, left ventricular end-diastolic diameter and ejection fraction in the cell transplantation group compared with the model group (P < 0.05). Hematoxylin-eosin staining showed that myocardial infarction was evident in the model group, in which, there were rarely viable myocardial tissues and few vessels in the infarcted region, but in the cell transplantation group, there were evident survived myocardial tissues and transplanted cells. The percentage of infarct size was significantly lower in the cell transplantation group than the model group (P < 0.05). Immunohistochemical staining showed that adipose-derived mesenchymal stem cells were able to survive in the infarcted myocardial tissues, and the expression of cardiac troponin T in the cell transplantation group was significantly higher than that in the model group (P < 0.05). Experimental data show that adipose-derived mesenchymal stem cell transplantation can protect the myocardial tissues after myocardial infarction, and effectively improve the myocardial function.